The Development of A Fluorescence Polarization Immunoassay for Aflatoxin Detection

被引：0
|
作者：
SHENG Ya Jie [1 ]
EREMIN Sergei [2 ]
MI Tie Jun [1 ]
ZHANG Su Xia [1 ]
SHEN Jian Zhong [1 ]
WANG Zhan Hui [1 ]
机构：
[1] Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, and Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety
[2] Department of Chemistry, M.V. Lomonosov Moscow State University
来源：
BiomedicalandEnvironmentalSciences | 2014年 / 27卷 / 02期
关键词：
FPIA; AFB; The Development of A Fluorescence Polarization Immunoassay for Aflatoxin Detection; AFM; EDF;
D O I：
暂无
中图分类号：
R114 [卫生毒理];
学科分类号：
100405 ;
摘要：
A fluorescence polarization immunoassay(FPIA)was developed for the analysis ofaflatoxins(AFs)using an anti-aflatoxin B1(AFB1)monoclonal antibody and a novel fluorescein-labeled AFB1tracer.The FPIA showed an IC50 value of 23.33ng/mL with a limit of detection of 13.12 ng/mL for AFB1.The cross-reactivities of AFB1,AFB2,AFG1,AFG2,AFM1,and AFM2 with the antibody were100%,65.7%,143%,23.5%,111.4%,and 2%,respectively.The group-specificity of anti-AFB1mAb indicated that the FPIA could potentially be used in a screening method for the detection of total AFs,albeit not AFG2 and AFM2.The total time required for analyzing 96 samples in one microplate was less than 5 min.This study demonstrates the potential usefulness of the FPIA as a rapid and simple technique for monitoring AFs.
引用
收藏
页码：126 / 129
页数：4
相关论文
共 50 条
  • [41] Development of one-step fluorescence polarization immunoassay for progesterone
    Hong, JY
    Choi, MJ
    BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2002, 25 (10) : 1258 - 1262
  • [42] Development of Fluorescence Polarization Immunoassay for Imidacloprid in Environmental and Agricultural Samples
    Zhou, Liangliang
    Yang, Jiachuan
    Tao, Zhexuan
    Eremin, Sergei A.
    Hua, Xiude
    Wang, Minghua
    FRONTIERS IN CHEMISTRY, 2020, 8
  • [43] Development of fluorescence polarization immunoassay for determination of pesticides and veterinary drugs
    Wang Zhan-hui
    Zhang Su-xia
    Shen Jian-zhong
    Sergei A Eremin
    SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27 (11) : 2299 - 2306
  • [44] Production of polyclonal antibodies and development of fluorescence polarization immunoassay for sulfanilamide
    Eremin, SA
    Murtazina, NR
    Ermolenko, DN
    Zherdev, AV
    Mart'ianov, AA
    Yazynina, EV
    Michura, IV
    Formanovsky, AA
    Dzantiev, BB
    ANALYTICAL LETTERS, 2005, 38 (06) : 951 - 969
  • [45] Development of an Ultrasensitive and Rapid Fluorescence Polarization Immunoassay for Ochratoxin A in Rice
    Huang, Xiaorong
    Tang, Xiaoqian
    Jallow, Abdoulie
    Qi, Xin
    Zhang, Wen
    Jiang, Jun
    Li, Hui
    Zhang, Qi
    Li, Peiwu
    TOXINS, 2020, 12 (11)
  • [46] Development of a rapid, specific fluorescence polarization immunoassay for the herbicide chlorsulfuron
    Eremin, SA
    Ryabova, IA
    Yakovleva, JN
    Yazynina, EV
    Zherdev, AV
    Dzantiev, BB
    ANALYTICA CHIMICA ACTA, 2002, 468 (02) : 229 - 236
  • [47] FLUORESCENCE POLARIZATION IMMUNOASSAY FOR CORTISOL
    KOBAYASHI, Y
    AMITANI, K
    WATANABE, F
    MIYAI, K
    CLINICA CHIMICA ACTA, 1979, 92 (02) : 241 - 247
  • [48] FLUORESCENCE POLARIZATION IMMUNOASSAY FOR ZIDOVUDINE
    GRANICH, GG
    EVELAND, MR
    KROGSTAD, DJ
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1989, 33 (08) : 1275 - 1279
  • [49] Fluorescence Polarization Immunoassay of Ractopamine
    Zvereva, E. A.
    Shpakova, N. A.
    Zherdev, A. V.
    Liu, L.
    Xu, C.
    Eremin, S. A.
    Dzantiev, B. B.
    APPLIED BIOCHEMISTRY AND MICROBIOLOGY, 2016, 52 (06) : 673 - 678
  • [50] Fluorescence polarization immunoassay of ractopamine
    E. A. Zvereva
    N. A. Shpakova
    A. V. Zherdev
    L. Liu
    C. Xu
    S. A. Eremin
    B. B. Dzantiev
    Applied Biochemistry and Microbiology, 2016, 52 : 673 - 678
12345