美洲大蠊多肽PAE2逆转BEL-7402/5-FU细胞多药耐药性的作用及机制研究

被引：5

作者：

王彦权 ^{[1
]}

李彩琳 ^{[1
]}

NAJIB Mohammerd ^{[1
]}

彭芳 ^{[1
]}

吴定宇 ^{[2
]}

机构：

[1] 大理大学药学院

[2] 大理大学实验室管理处

来源：

中国医药工业杂志 | 2021年 / 52卷 / 01期

关键词：

人肝细胞肿瘤; 多药耐药; 交叉耐药; 索拉非尼; 美洲大蠊多肽PAE2;

D O I：

10.16522/j.cnki.cjph.2021.01.007

中图分类号：

R285 [中药药理学];

学科分类号：

摘要：

本研究探讨了美洲大蠊多肽PAE2逆转人肝细胞肿瘤耐药细胞株BEL-7402/5-FU的多药耐药性作用及机制。以人肝细胞肿瘤敏感细胞株BEL-7402、人肝细胞肿瘤耐5-氟尿嘧啶（5-FU）细胞株BEL-7402/5-FU和Balb/c-nude小鼠为研究对象,采用噻唑蓝比色分析法检测BEL-7402/5-FU细胞的多药耐药性和交叉耐药性,通过激光共聚焦显微镜观察阿霉素在BEL-7402/5-FU细胞中的累积。使用实时荧光定量聚合酶链式反应和免疫细胞化学染色法检测BEL-7402/5-FU细胞中P-糖蛋白1（P-glycoprotein 1,P-gp1）、多药耐药相关蛋白1（multi-drug resistance protein 1,MRP1）、肺耐药相关蛋白1（lung resistance protein 1,LRP1）以及乳腺癌耐药蛋白1（breast cancer resistance protein 1,BCRP1）的mRNA和蛋白的相对表达量,确定美洲大蠊多肽PAE2逆转多药耐药性的机制。此外,在Balb/c-nude小鼠中,建立BEL-7402/5-FU细胞的腋下移植瘤模型,观察美洲大蠊多肽PAE2对腋下移植瘤小鼠的免疫器官、肝肾功能、肿瘤抑制与诱导肿瘤细胞调亡、调节肿瘤组织多药耐药相关蛋白等环节的影响。结果显示,美洲大蠊多肽PAE2能有效逆转BEL-7402/5-FU细胞的多药耐药性和交叉耐药性,作用机制可能与下调多药耐药相关蛋白的表达水平有关,降低了多药耐药细胞对抗肿瘤药物的外排能力、影响抗肿瘤药物分子代谢,从而达到逆转人肝细胞癌多药耐药的作用。

引用

页码：68 / 79

页数：12

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