Oral polymorphonuclear neutrophil characteristics in relation to oral health: a cross-sectional, observational clinical study

被引:1
|
作者
Patrick Rijkschroeff [1 ]
Ineke DC Jansen [1 ]
Fridus A van der Weijden [1 ]
Bart JF Keijser [2 ,3 ,4 ]
Bruno G Loos [1 ]
Elena A Nicu [1 ]
机构
[1] Department of Periodontology,Academic Centre for Dentistry Amsterdam
[2] Department of Preventive Dentistry,Academic Centre for Dentistry Amsterdam
[3] Microbiology and Systems Biology,TNO Earth,Environmental and Life Sciences  4. Top Institute Food and Nutrition
关键词
apoptosis; degranulation; neutrophils; oral health; reactive oxygen species;
D O I
暂无
中图分类号
R780.1 [口腔疾病的预防与卫生];
学科分类号
1003 ;
摘要
Polymorphonuclear neutrophils(PMNs) have a major role in the innate immune system. However, little is known about PMN contribution in relation to oral health. The objective of this study was to investigate the numbers and functional characteristics of oral PMNs(o PMNs) compared with circulatory PMNs(c PMNs). Oral rinse and venous blood samples were obtained from 268 systemically and orally healthy volunteers in a cross-sectional observational study. PMN counts, cell cycle analysis and cellular activation state were investigated. Also, reactive oxygen species(ROS) production was analyzed, with and without bacterial stimulation(Fusobacterium nucleatum). In males, 1.2 × 106± 1.0 × 106oPMNs were collected, and showed a tendency to correlate with the levels of gingival bleeding(r = 0.215, P = 0.008). Comparable o PMNs counts were found among females(1.0 × 106± 0.7 × 106). More late-stage apoptotic/necrotic cells were found among the o PMNs(53.1%) compared with the c PMNs(8.5%; Po0.001). Without additional stimulation, o PMNs were more activated than c PMNs, as indicated by higher expression of CD11 b, CD63 and CD66 b, and higher constitutive ROS levels(Po0.001). Notably, in response to bacterial stimulation, o PMNs released comparable ROS levels as c PMNs(P = 0.042). In conclusion, this study provides data on viable o PMNs showing high levels of activation in orally and systemically healthy individuals, free of apparent caries lesions and periodontal disease. These data suggests that although the o PMNs are in a more mature stage of their life cycle compared with the c PMNs, o PMNs are still responsive to stimulation, which indicates their functional potential and possible contribution to a healthy oral ecosystem.
引用
收藏
页码:191 / 198
页数:8
相关论文
共 50 条
  • [21] The Association of Dietary Intake, Oral Health, and Blood Pressure in Older Adults: A Cross-Sectional Observational Study
    Marito, Pinta
    Hasegawa, Yoko
    Tamaki, Kayoko
    Maria, Ma Therese Sta
    Yoshimoto, Tasuku
    Kusunoki, Hiroshi
    Tsuji, Shotaro
    Wada, Yosuke
    Ono, Takahiro
    Sawada, Takashi
    Kishimoto, Hiromitsu
    Shinmura, Ken
    NUTRIENTS, 2022, 14 (06)
  • [22] Assessment of the oral health literacy and oral health behaviors among nurses in China: a cross-sectional study
    An, Ran
    Chen, Wen-feng
    Li, Siyu
    Wu, Zitong
    Liu, Meizi
    Sohaib, Muhammad
    BMC ORAL HEALTH, 2022, 22 (01)
  • [23] Assessment of the oral health literacy and oral health behaviors among nurses in China: a cross-sectional study
    Ran An
    Wen-feng Chen
    Siyu Li
    Zitong Wu
    Meizi Liu
    Muhammad Sohaib
    BMC Oral Health, 22
  • [24] Characteristics of oral health literacy in Senegal: A cross-sectional study among women in the Department of Pikine
    Dieng, Serigne Ndame
    Kanoute, Aida
    Lombrail, Pierre
    Diouf, Massamba
    Azogui-Levy, Sylvie
    JOURNAL OF PUBLIC HEALTH IN AFRICA, 2022, 13 (02)
  • [25] Cross-sectional study of oral health care service, oral health beliefs and oral health care education of caregivers in nursing homes
    Sigurdardottir, Adalheidur Svana
    Geirsdottir, Olof Gudny
    Ramel, Alfons
    Arnadottir, Inga Bergmann
    GERIATRIC NURSING, 2021, 43 : 138 - 145
  • [26] The symptom network of oral health conditions in older populations with oral frailty: a cross-sectional study
    Zhang, Chengrui
    Ying, Xing
    Wei, Luan
    Bin, Chen
    BMC ORAL HEALTH, 2025, 25 (01):
  • [27] Oral Health Knowledge, Attitudes, and Clinical Practices of Pediatricians and Pediatric Residents: A Cross-Sectional Study
    Farsi, Deema
    Alagili, Dania
    CUREUS JOURNAL OF MEDICAL SCIENCE, 2023, 15 (12)
  • [28] Implications of β-thalassemia on oral health status in patients: A cross-sectional study
    Nabi, Aaysha T.
    Muttu, Jayalakshmi
    Chhaparwal, Amit
    Mukhopadhyay, Arka
    Pattnaik, Samarjeet J.
    Choudhary, Pallawee
    JOURNAL OF FAMILY MEDICINE AND PRIMARY CARE, 2022, 11 (03) : 1174 - 1178
  • [29] Association between oral health and bowel habits: a cross-sectional study
    Zuhong Ji
    Jianwen Mei
    Youjian Li
    Zijie Wang
    Zhirui Guo
    Lin Miao
    BMC Public Health, 25 (1)
  • [30] Oral health status of adult hypophosphatasia patients: A cross-sectional study
    Weider, Margareta
    Schlagenhauf, Ulrich
    Seefried, Lothar
    JOURNAL OF CLINICAL PERIODONTOLOGY, 2022, 49 (12) : 1253 - 1261