Expression of differential nitric oxide synthase isoforms in human gastric mucosa infected with Helicobacter pylori

被引：0
|
作者：
屠振兴
龚燕芳
丁华
许国铭
李兆申
满晓华
机构：
[1] Changhai Hospital
[2] Second Military Medical University
[3] Shanghai 200433
[4] China
[5] Department of Gastroenterology
来源：
Journal of Medical Colleges of PLA | 2003年 / 05期
基金：
中国国家自然科学基金;
关键词：
H; pylori; nitric oxide synthase; immunohistochemistry;
D O I：
暂无
中图分类号：
R573 [胃疾病];
学科分类号：
1002 ; 100201 ;
摘要：
Objective: To study the relationship between nitric oxide synthase ( NOS) expression in human gastric mu-cosa and Helicobacter pylori (H. pylori) infection. Methods: Gastric mucosa samples were obtained from antrum of 33 patients received gastroendoscopy. H. pylori infection was confirmed by Giems staining and bacteria culture under mi-croaerophilic conditions. Expression of iNOS, eNOS and nitrotyrosine were detected by immunohistochemistry. Results: (1) The positive rate of H. pylori infection was 66. 7% (22/33) . (2) iNOS positive staining in inflammatory cells was detected in 77. 3% (17/22) of samples with H. pylori and 27. 3% (3/11) without H. pylori infection (P < 0.01). (3) eNOS expression in inflammatory cells was found in 77. 3% (17/22) of samples with H. pylori and 18.2% (2/11) without H. pylori infection ( P < 0. 01) . (4) Nitrotyrosine expression in inflammatory cells was observed in 59. 1% ( 13/22) of samples with H. pylori and 54.5%(6/11) without H. pylori infection (P>0.05). (5) Moderate a
引用
收藏
页码：286 / 289
页数：4
相关论文
共 50 条
  • [31] No Additive Effect Between Helicobacter pylori Infection and Portal Hypertensive Gastropathy on Inducible Nitric Oxide Synthase Expression in Gastric Mucosa of Cirrhotic Patients
    Usama A. Arafa
    Yasuhiro Fujiwara
    Kazuhide Higuchi
    Masatsugu Shiba
    Toshiyuki Uchida
    Toshio Watanabe
    Kazunari Tominaga
    Nobuhide Oshitani
    Takayuki Matsumoto
    Tetsuo Arakawa
    Digestive Diseases and Sciences, 2003, 48 : 162 - 168
  • [32] No additive effect between Helicobacter pylori infection and portal hypertensive gastropathy on inducible nitric oxide synthase expression in gastric mucosa of cirrhotic patients
    Arafa, UA
    Fujiwara, Y
    Higuchi, K
    Shiba, M
    Uchida, T
    Watanabe, T
    Tominaga, K
    Oshitani, N
    Matsumoto, T
    Arakawa, T
    DIGESTIVE DISEASES AND SCIENCES, 2003, 48 (01) : 162 - 168
  • [33] Influence of Helicobacter pylori (Hp) colonization on nitric oxide synthase (NOS) activity and levels of cGMP in gastric mucosa.
    Esteban, J
    Rodriguez, MA
    Pellicer, F
    Hergueta, P
    Delgado, D
    Herrerias, J
    GASTROENTEROLOGY, 1996, 110 (04) : A104 - A104
  • [34] p53 expression in the gastric mucosa of patients infected with Helicobacter pylori
    Morales-Fuentes, G. A.
    Zarate-Osorno, A.
    Quinonez-Urrego, E. E.
    Antonio-Manrique, M.
    Martinez-Garcia, C. L.
    Figueroa-Barojas, P.
    Zamorano-Orozco, Y.
    Leal-Osuna, S. E.
    Martinez-Camacho, C.
    Mejia-Cuan, L. A.
    Rivera-Nava, C. A.
    Sanchez-Chavez, X.
    Ramirez-Ramirez, M. A.
    REVISTA DE GASTROENTEROLOGIA DE MEXICO, 2013, 78 (01): : 12 - 20
  • [35] P53 Expression in Gastric Mucosa of Patients Infected With Helicobacter pylori
    Morales-Fuentes, Gerardo A.
    Barojas, Paola Figueroa
    Antonio-Manrique, Martin
    Luz, Martinez G. Clara
    Zamorano, Yolanda
    Zarate-Osorno, Alejandra
    Quinonez-Urrego, Enoe E.
    Mejia-Cuan, Luis Alvaro
    Martinez-Camacho, Claudia
    Rivera-Nava, Cesar A.
    Sanchez-Chavez, Xochiquetzal
    Rojas-Macuil, Paulo
    Montenegro-Molina, William
    Leal-Osuna, Sergio Enrique
    Ramirez-Ramirez, Miguel A.
    GASTROENTEROLOGY, 2012, 142 (05) : S476 - S476
  • [36] Nitric oxide synthase activity and expression its isoforms in gastric mucosa under stress-induced ulceration
    Maksymovych, I.
    Drobinska, O.
    Ostapchenko, L.
    FEBS JOURNAL, 2010, 277 : 136 - 137
  • [37] NO homeostasis in Helicobacter pylori-infected gastric mucosa
    Miura, S
    JOURNAL OF GASTROENTEROLOGY, 1999, 34 (06) : 717 - 720
  • [38] NO homeostasis in Helicobacter pylori-infected gastric mucosa
    Journal of Gastroenterology, 1999, 34 (6) : 717 - 720
  • [39] Expression of nitric oxide synthase isoforms in human liver cirrhosis
    Mohammed, NA
    El-Aleem, SA
    Appleton, I
    Maklouf, MM
    Said, M
    McMahon, RF
    JOURNAL OF PATHOLOGY, 2003, 200 (05): : 647 - 655
  • [40] Expression of melanoma differentiation associated gene 5 is increased in human gastric mucosa infected with Helicobacter pylori
    Tatsuta, Tetsuya
    Imaizumi, Tadaatsu
    Shimoyama, Tadashi
    Sawaya, Manabu
    Kunikazu, Tanji
    Matsumiya, Tomoh
    Yoshida, Hidemi
    Satoh, Kei
    Fukuda, Shinsaku
    JOURNAL OF CLINICAL PATHOLOGY, 2012, 65 (09) : 839 - 843
12345