Endonuclease modified comet assay for oxidative DNA damage induced by detection of genetic toxiants

被引：0
|
作者：
赵健 [1 ]
机构：
[1] Key Lab Chem Safe & Health,Chin Dis Contr & Prev Center
来源：
ChinaMedicalAbstracts(InternalMedicine) | 2014年 / 31卷 / 02期
关键词：
Endonuclease modified comet assay for oxidative DNA damage induced by detection of genetic toxiants; DNA;
D O I：
暂无
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
Objective The aim of this study was to investigate the use of the lesion-specific endonucleases-modifiedcomet assay for analysis of DNA,oxidation in cell lines.Methods DNA breaks and oxidative damage were evaluated by normal alkaline and formamidopyrimidine-DNAglycosylase(FPG)modified comet assays.Cytotoxicity was assessed by MTT method.The human bronchial epi-
引用
收藏
页码：66 / 66
页数：1
相关论文
共 50 条
  • [31] Cytogenetic status and oxidative DNA-damage induced by atorvastatin in human peripheral blood lymphocytes: Standard and Fpg-modified comet assay
    Gajski, Goran
    Garaj-Vrhovac, Vera
    Orescanin, Visnja
    TOXICOLOGY AND APPLIED PHARMACOLOGY, 2008, 231 (01) : 85 - 93
  • [32] Detection of DNA damage in haemocytes of zebra mussel using comet assay
    Pavlica, M
    Klobucar, GIV
    Mojas, N
    Erben, R
    Papes, D
    MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2001, 490 (02) : 209 - 214
  • [33] Detection of DNA damage by alkaline comet assay in mouse colonic mucosa
    Boutet-Robinet, Elisa
    Haykal, Maria M.
    Hashim, Saleha
    Frisan, Teresa
    Martin, Oceane C. B.
    STAR PROTOCOLS, 2021, 2 (04):
  • [34] Detection of oxidative DNA damage in isolated marine bivalve hemocytes using the comet assay and formamidopyrimidine glycosylase (Fpg)
    Gielazyn, ML
    Ringwood, AH
    Piegorsch, WW
    Stancyk, SE
    MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2003, 542 (1-2) : 15 - 22
  • [35] Heterogeneity reflected in the comet assay -: Studies on heterogeneity of radiation-induced DNA damage using the comet assay
    Seidel, C
    Müller, AC
    STRAHLENTHERAPIE UND ONKOLOGIE, 2004, 180 : 64 - 64
  • [36] Comet assay to determine DNA damage induced by food deprivation in rats
    Rocha, Gabrielle De Souza
    Fonseca, A. S.
    Rodrigues, Michelle P.
    Dantas, F. J. S.
    Caldeira-De-Araujo, A.
    Santos, R.
    ACTA BIOLOGICA HUNGARICA, 2008, 59 (03): : 315 - 325
  • [37] Overestimation of nanoparticles-induced DNA damage determined by the comet assay
    Ferraro, Daniela
    Anselmi-Tamburini, Umberto
    Tredici, Ilenia Giuseppina
    Ricci, Vittorio
    Sommi, Patrizia
    NANOTOXICOLOGY, 2016, 10 (07) : 861 - 870
  • [38] Diagnosis of Metals Induced DNA Damage in Fish Using Comet Assay
    Kousar, Safina
    Javed, Muhammad
    PAKISTAN VETERINARY JOURNAL, 2015, 35 (02) : 168 - 172
  • [39] DNA damage induced by copper deficiency in cattle assessed by the Comet assay
    Picco, SJ
    De Luca, JC
    Mattioli, G
    Dulout, FN
    MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2001, 498 (1-2) : 1 - 6
  • [40] Induced DNA damage measured by the Comet assay in 10 weed species
    Gichner, T
    Mühlfeldová, Z
    BIOLOGIA PLANTARUM, 2002, 45 (04) : 509 - 516
12345