Genetic diversity in Penaeus chinensis shrimp as revealed by RAPD technique

The random amplified polymorphic DNA analysis was used to estimate genetic diversity in one successively cultivated stock and three wild stocks of Penaeus chinensis shrimp, two of which were collected from the spawning and wintering grounds in the west coast of Korean Peninsula, and one from the feeding ground in the China coast of the Yellow Sea. A random primer kit was employed to scan the genomic DNA in 20 individuals of each index stock. A total of 110 reproducible RAPD markers were obtained, 68.2% of which showed a sound conformability within all the individuals detected, implying that the genetic variability in P. chinensis is relatively low. The proportions of polymorphic loci among these four stocks ranged from 20% to 33.3% , while the degrees of genetic polymorphisms varied from 0.0093 to 0.0307. The genetic variability of inter- stocks was higher than that of intra- stock. The genetic diversity in different stocks differed from each other; that is, a less genetic differentiation in the spawnin