Preparation and Characterization of Monoclonal Antibodies against VP1 Protein of Foot-and-mouth Disease Virus O/China99

被引：0
|
作者：
Shuai SONG1
2.Guangdong Open Laboratory of Veterinary Public Healty
机构：
来源：
Virologica Sinica | 2009年 / 24卷 / 06期
基金：
国家高技术研究发展计划(863计划);
关键词：
Foot-and-mouth disease virus (FMDV); Monoclonal antibody; Neutralizing activity; VP1; protein;
D O I：
暂无
中图分类号：
R373 [人体病毒学（致病病毒）];
学科分类号：
100103 ; 100705 ;
摘要：
Monoclonal antibodies (McAbs) 1A9 and 9F12 against Foot-and-mouth disease virus (FMDV) serotype O were produced by fusing SP2/0 myeloma cells with splenocyte from the mouse immunized with O/China99. Both McAbs reacted with O/China99 but not with Asia 1, as determined by immunohistochemistry assay. The microneutralization titer of the McAbs 1A9 and 9F12 were 640 and 1 280, respectively. Both McAbs contain kappa light chains, but the McAbs 1A9 and 9F12 were IgG1 and IgM, respectively. In order to define the McAbs binding epitopes, the reactivity of these McAbs against VP1, P20 and P14 were examined using indirect ELISA, the result showed that both McAbs reacted with VP1 and P20. McAbs may be used for further studies of vaccine, diagnostic methods, prophylaxis, etiological and immunological researches on FMDV.
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收藏
页码：566 / 572
页数：7
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