CRISPR/Cas9-Mediated Adenine Base Editing in Rice Genome

被引：0

作者：

LI Hao ^{[1
]}

QIN Ruiying ^{[1
]}

LIU Xiaoshuang ^{[1
]}

LIAO Shengxiang ^{[1
]}

XU Rongfang ^{[1
]}

YANG Jianbo ^{[1
]}

WEI Pengcheng ^{[1
]}

机构：

[1] Key Laboratory of Rice Genetics & Breeding,Institute of Rice Research,Anhui Academy of Agricultural Science

来源：

Rice Science | 2019年 / 02期

基金：

中国国家自然科学基金;

关键词：

ABE; CRISPR/Cas9-Mediated Adenine Base Editing in Rice Genome;

D O I：

暂无

中图分类号：

S511 [稻];

学科分类号：

0901 ;

摘要：

Precise base editing is highly desired in plant functional genomic research and crop molecular breeding. In this study, we constructed a rice-codon optimized adenine base editor(ABE)-nC as9 tool that induced targeted A·T to G·C point mutation of a key single nucleotide polymorphism site in an important agricultural gene. Combined with the modified single-guide RNA variant, our plant ABE tool can efficiently achieve adenine base editing in the rice genome.

引用

页码：125 / 128

页数：4

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