Sequence of rDNA intergenic spacer region (ISR) from a waterbloom cyanobacterial species Oscilla-toria sp. was determined and analyzed. The results of sequence comparison showed that the spacer had a high level sequence divergence, suggesting the sequence may be a target sequence for developing cyanobacteria genus- and species-specific oligonucleotide probes. In addition, a 20bp sequence of rDNA ISR was found highly conserved in all species of cyanobacteria, which was not found in other eubacteria. This conserved sequence within a variable region indicates that it might be a functional oligonucleotide in the processing of the rRNA precursor.
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Zhongshan Univ, Sch Life Sci, State Key Lab Biocontrol, Canton 510275, Peoples R ChinaZhongshan Univ, Sch Life Sci, State Key Lab Biocontrol, Canton 510275, Peoples R China
Chen, YQ
He, JW
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机构:Zhongshan Univ, Sch Life Sci, State Key Lab Biocontrol, Canton 510275, Peoples R China
He, JW
Tang, SQ
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机构:Zhongshan Univ, Sch Life Sci, State Key Lab Biocontrol, Canton 510275, Peoples R China
Tang, SQ
Zhuang, L
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机构:Zhongshan Univ, Sch Life Sci, State Key Lab Biocontrol, Canton 510275, Peoples R China
Zhuang, L
Qu, LH
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机构:Zhongshan Univ, Sch Life Sci, State Key Lab Biocontrol, Canton 510275, Peoples R China
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Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki 3058572, JapanUniv Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki 3058572, Japan
Uzuhashi, Shihomi
Imazu, Michio
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Shinshu Univ, Sch Gen Educ, Nagano, JapanUniv Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki 3058572, Japan
Imazu, Michio
Kakishima, Makoto
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Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki 3058572, JapanUniv Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki 3058572, Japan
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Tel Aviv Univ, Raymond & Beverly Sackler Fac Exact Sci, Sch Chem, IL-69978 Tel Aviv, IsraelTel Aviv Univ, Raymond & Beverly Sackler Fac Exact Sci, Sch Chem, IL-69978 Tel Aviv, Israel
Banker, R
Carmeli, S
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Tel Aviv Univ, Raymond & Beverly Sackler Fac Exact Sci, Sch Chem, IL-69978 Tel Aviv, IsraelTel Aviv Univ, Raymond & Beverly Sackler Fac Exact Sci, Sch Chem, IL-69978 Tel Aviv, Israel