Time-resolved ESI-MS Investigation on Reduction and Alkylation in Unfolding Process of Lysozyme

被引：0
|
作者：
SONG Hao-wei1
2. State Key Laboratory of Genetic Engineering
机构：
来源：
Chemical Research in Chinese Universities | 2001年 / 04期
关键词：
Keywords Unfolding; Lysozyme; Reduction and alkylation; Time-resolved ESI-MS;
D O I：
暂无
中图分类号：
O621 [有机化学一般性问题];
学科分类号：
070303 ; 081704 ;
摘要：
The kinetic procedure of the unfolding of lysozyme induced by the reduction of disulfide was monitored by the time-resolved ESI-MS with a sheath liquid assistant electrospray interface. It was found that the reduction process for the eight disulfides had a less difference in the reaction time after denatured treatment. In addition, the alkylation of the reduced free thiols was much slower than the reduction procedure. An artifact peak produced by the CID fragmentation in the mass spectra was identified and the possible mechanism of the Hofmann elimination reaction was proposed.
引用
收藏
页码：387 / 393
页数：7
相关论文
共 50 条
  • [1] Time-resolved ESI-MS investigation on reduction and alkylation in unfolding process of lysozyme
    Song, HW
    Xu, CF
    Fu, YL
    Yang, PY
    Wang, HH
    CHEMICAL RESEARCH IN CHINESE UNIVERSITIES, 2001, 17 (04) : 387 - 393
  • [2] Conformational dynamics of myoglobin studied by time-resolved ESI-MS and on-line H/D exchange.
    Simmons, DA
    Konermann, L
    BIOPHYSICAL JOURNAL, 2003, 84 (02) : 156A - 156A
  • [3] ESI-MS investigation of the polymerization of inorganic polymers
    Simonsen, Morten E.
    Sogaard, Erik G.
    INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2009, 285 (1-2) : 78 - 85
  • [4] A mechanistic investigation of hydrodehalogenation using ESI-MS
    Ahmadi, Zohrab
    McIndoe, J. Scott
    CHEMICAL COMMUNICATIONS, 2013, 49 (98) : 11488 - 11490
  • [5] Degradation process of bioresorbable copolyesters. Microstructure investigation by NMR and ESI-MS
    Jaworska, Joanna
    Kasperczyk, Janusz
    Dobrzynski, Piotr
    MACROMOLECULAR SYMPOSIA, 2007, 253 : 40 - 46
  • [6] Interaction Between N-Phosphoryl Dopamines and Lysozyme by ESI-MS
    Wu Xian-Li
    Han Guo-Sheng
    Wang Zhen
    Cao Shu-Xia
    Liao Xin-Cheng
    CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE, 2012, 33 (03): : 516 - 520
  • [7] Investigation of radical cation in electrophilic fluorination by ESI-MS
    Zhang, X
    Liao, YX
    Qian, R
    Wang, HY
    Guo, YL
    ORGANIC LETTERS, 2005, 7 (18) : 3877 - 3880
  • [8] Studies on the Basic Conformation of Lysozyme by Electrospray Ionization-Mass Spectrometry(ESI-MS)
    GU Sheng
    ChemicalResearchinChineseUniversities, 2001, (04) : 368 - 373
  • [9] Studies on the basic conformation of lysozyme by electrospray ionization-mass spectrometry (ESI-MS)
    Gu, S
    Shen, JC
    Zhuang, ZX
    Wang, XR
    CHEMICAL RESEARCH IN CHINESE UNIVERSITIES, 2001, 17 (04) : 368 - 373
  • [10] Reduction of signal suppression effects in ESI-MS using a nanosplitting device
    Gangl, ET
    Annan, M
    Spooner, N
    Vouros, P
    ANALYTICAL CHEMISTRY, 2001, 73 (23) : 5635 - 5644
12345