Educational university context represents a series of challenges that can affect students' physical and psychological health. This highlights the need to focus on the empowerment of one's own resources, which allow them to assess experiences in a positive way and increase satisfaction with life aspects. Thus, the aim of this research is to analyze the incidence of an emotional physical education program on the subjective well-being of university students in initial teacher training, considering the cognitive and affective-emotional components. A quasi-experimental with mixed methodology of convergent design or recurrent triangulation, was carried out with the participation of 229 students from two Chilean universities. To assess the cognitive component, the Satisfaction with Life Scale (SWLS) was used; performing pretest and posttest mean difference, with control group and experimental group. To measure the affective-emotional component, a Focus Group information production technique was used with the experimental group. The results confirm that there are significant differences in pretest and posttest values between the experimental group and the control group. The students, in turn, perceive an increase in their emotional competences and positive feelings on a daily basis. Therefore, this study shows the positive impact of an emotional physical education program on the subjective well-being of university students.