Insights into the subdaily variations in methane, nitrous oxide and carbon dioxide fluxes from upland tropical tree stems

Recent studies have shown that stem fluxes, although highly variable among trees, can alter the strength of the methane (CH4) sink or nitrous oxide (N2O) source in some forests, but the patterns and magnitudes of these fluxes remain unclear. This study investigated the drivers of subdaily and seasonal variations in stem and soil CH4, N2O and carbon dioxide (CO2) fluxes. CH4, N2O and CO2 fluxes were measured continuously for 19 months in individual stems of two tree species, Eperua falcata (Aubl.) and Lecythis poiteaui (O. Berg), and surrounding soils using an automated chamber system in an upland tropical forest. Subdaily variations in these fluxes were related to environmental and stem physiological (sap flow and stem diameter variations) measurements under contrasting soil water conditions. The results showed that physiological and climatic drivers only partially explained the subdaily flux variations. Stem CH4 and CO2 emissions and N2O uptake varied with soil water content, time of day and between individuals. Stem fluxes decoupled from soil fluxes. Our study contributes to understanding the regulation of stem greenhouse gas fluxes. It suggests that additional variables (e.g. internal gas concentrations, wood-colonising microorganisms, wood density and anatomy) may account for the remaining unexplained variability in stem fluxes, highlighting the need for further studies.