Top-down mass spectrometry of native proteoforms and their complexes: a community study

被引：7

作者：

Habeck, Tanja ^{[1
]}

Brown, Kyle A. ^{[2
]}

Des Soye, Benjamin ^{[3
]}

Lantz, Carter ^{[4
]}

Zhou, Mowei ^{[5
,11
]}

Alam, Novera ^{[6
]}

Hossain, Md Amin ^{[6
]}

Jung, Wonhyeuk ^{[4
]}

Keener, James E. ^{[7
]}

Volny, Michael ^{[8
]}

Wilson, Jesse W. ^{[5
]}

Ying, Yujia ^{[9
]}

Agar, Jeffrey N. ^{[6
,10
]}

Danis, Paul O. ^{[10
]}

Ge, Ying ^{[2
,10
]}

Kelleher, Neil L. ^{[3
,10
]}

Li, Huilin ^{[9
]}

Loo, Joseph A. ^{[4
,10
]}

Marty, Michael T. ^{[7
]}

Pasa-Tolic, Ljiljana ^{[5
,10
]}

Sandoval, Wendy ^{[8
]}

Lermyte, Frederik ^{[1
]}

机构：

[1] Tech Univ Darmstadt, Darmstadt, Germany

[2] Univ Wisconsin Madison, Madison, WI USA

[3] Northwestern Univ, Evanston, IL USA

[4] Univ Calif Los Angeles, Los Angeles, CA USA

[5] Pacific Northwest Natl Lab, Richland, WA USA

[6] Northeastern Univ, Boston, MA USA

[7] Univ Arizona, Tucson, AZ USA

[8] Genentech Inc, San Francisco, CA USA

[9] Sun Yat Sen Univ, Guangzhou, Peoples R China

[10] Consortium Top Down Proteom, Cambridge, MA USA

[11] Zhejiang Univ, Zhejiang, Peoples R China

来源：

NATURE METHODS | 2024年 / 21卷 / 12期

基金：

美国国家卫生研究院;

关键词：

PROTEIN COMPLEXES; ION MOBILITY; MEMBRANE-PROTEINS; SOFTWARE TOOL; PROTEOMICS; RESOLUTION; SPECTRA; FRAGMENTATION; DECONVOLUTION; SENSITIVITY;

D O I：

10.1038/s41592-024-02279-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The combination of native electrospray ionization with top-down fragmentation in mass spectrometry (MS) allows simultaneous determination of the stoichiometry of noncovalent complexes and identification of their component proteoforms and cofactors. Although this approach is powerful, both native MS and top-down MS are not yet well standardized, and only a limited number of laboratories regularly carry out this type of research. To address this challenge, the Consortium for Top-Down Proteomics initiated a study to develop and test protocols for native MS combined with top-down fragmentation of proteins and protein complexes across 11 instruments in nine laboratories. Here we report the summary of the outcomes to provide robust benchmarks and a valuable entry point for the scientific community.

引用

页码：2388 / 2396

页数：14

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