Biomimetic Recognition of SARS-CoV-2 Receptor-Binding Domain N-Glycans by an Antiviral Synthetic Receptor

被引：0

作者：

Santambrogio, Carlo ^{[1
]}

Toccafondi, Mirco ^{[2
]}

Donnici, Lorena ^{[2
]}

Pesce, Elisa ^{[2
,3
]}

De Francesco, Raffaele ^{[2
,4
]}

Grifantini, Renata ^{[2
]}

Ponzini, Erika ^{[5
,6
]}

Milanesi, Francesco ^{[7
]}

Fragai, Marco ^{[8
]}

Nativi, Cristina ^{[7
]}

Roelens, Stefano ^{[7
,9
]}

Grandori, Rita ^{[1
,10
]}

Francesconi, Oscar ^{[7
]}

机构：

[1] Univ Milano Bicocca, Dipartimento Biotecnol & Biosci, I-20126 Milan, Italy

[2] Ist Nazl Genet Mol INGM Romeo & Enrica Invernizzi, I-20122 Milan, Italy

[3] Univ Milan, Dipartimento Sci Clin & Comun, Dipartimento Eccellenza 2023 2027, Milan, Italy

[4] Univ Milan, Dipartimento Sci Farmacolog & Biomolecolari, Milan, Italy

[5] Univ Milano Bicocca, Dipartimento Sci Mat, I-20125 Milan, Italy

[6] Univ Milano Bicocca, Opt & Optometry Res Ctr COMiB, I-20125 Milan, Italy

[7] Univ Firenze, Dipartimento Chim Ugo Schiff DICUS, Florence, Italy

[8] Univ Firenze, Ctr Risonanze Magnet CERM, Florence, Italy

[9] Consorzio Interuniv Nazl Sci & Tecnol Mat INSTM, Florence, Italy

[10] Forschungszentrum Juelich, Inst Adv Simulat, D-52428 Julich, Germany

来源：

CHEMBIOCHEM | 2025年

关键词：

enveloped viruses; N-glycans; mannose recognition; synthetic receptors; native mass spectroscopy; TRIPODAL RECEPTORS; CAGE RECEPTOR; DC-SIGN; GLYCOSYLATION; HIV-1; SPIKE; GLYCOPROTEINS; PROTEINS; POTENT; VIRUS;

D O I：

10.1002/cbic.202500106

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recognition of glycans by simple synthetic receptors is a key issue in supramolecular chemistry, endowed with relevant implications in glycobiology and medicine. In this context, glycoproteins featuring N-glycans represent an important biological target, because they are often exploited by enveloped viruses in adhesion and infection processes. However, a direct evidence for their recognition by a synthetic receptor targeting N-glycans is still missing in the literature. Using a combination of glycoengineering and mass spectrometry techniques, we present here the direct evidence of biomimetic recognition of complex-type N-glycans exposed on the receptor-binding domain (RBD) of the wild-type spike protein of SARS-CoV-2 by a biologically active, synthetic receptor.

引用

页数：8

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