Cell Culture of a Swine Genotype 4 Hepatitis E Virus Strain

被引：1
|
作者：
Gong, Ga [1 ]
Xin, Jiaojiao [1 ]
Lou, Yongzhi [1 ]
Qiong, Da [1 ]
Dawa, Zhuoma [2 ]
Gesang, Zhuoma [3 ]
Suolang, Sizhu [1 ]
机构：
[1] Xizang Agr & Anim Husb Univ, Anim Sci Coll, Nyingchi, Peoples R China
[2] Vet Stn, Lhasa, Peoples R China
[3] Anim Dis Prevent & Control Ctr Xizang Autonomous R, Lhasa, Peoples R China
来源：
JOURNAL OF MEDICAL VIROLOGY | 2024年 / 96卷 / 11期
关键词：
cell culture; GT4; hepatitis E virus; swine;
D O I：
10.1002/jmv.70031
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
HEV infection has become a global health concern. The study of HEV pathogenicity has been hindered by the lack of a suitable in vitro culture system. In the present research, we systematic demonstration of efficient replication of swine GT4 HEV in A549 cells, Huh-7 cells, and HepG2/C3A cell lines. The results of the immunofluorescence assay and immunofluorescence confocal assay showed that swine GT4 HEV is efficiently replicated in three cell lines at 72 h postinoculation. Meanwhile, we also detected the virus titer quantified were increased at 2-, 6,- and 11-days postinoculation. Moreover, we successfully observed HEV virus particles in the cell suspension at 6 days postinoculation. This finding holds significance for advancing in vitro HEV studies.
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页数：6
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