Protocol to evaluate virus-specific CD4+ and CD8+ T cell responses by spectral flow cytometry

被引：0

作者：

Zhao, Xin-Jing ^{[1
,2
]}

Zhang, Sheng ^{[1
]}

Wei, Wang-Qian ^{[1
]}

Xu, Qiang ^{[1
]}

Lv, Chen-Long ^{[1
]}

Wang, Guo-Lin ^{[1
]}

Fang, Li-Qun ^{[1
,2
]}

机构：

[1] Acad Mil Med Sci, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China

[2] Anhui Med Univ, Sch Publ Hlth, Dept Epidemiol & Biostat, Hefei 230000, Peoples R China

来源：

STAR PROTOCOLS | 2024年 / 5卷 / 04期

基金：

中国国家自然科学基金; 北京市自然科学基金;

关键词：

D O I：

10.1016/j.xpro.2024.103497

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

T cell responses play an important role in viral clearance and prevention of infection. Here, we present a protocol to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific T cell responses using spectral flow cytometry. We describe steps for peripheral blood mononuclear cell (PBMC) thawing and stimulation with SARS-CoV-2 spike peptide pools. We then describe detailed procedures for cell confining, staining, and fixation. This protocol has potential application in spectral flow cytometry on other virus-specific T cell responses. For complete details on the use and execution of this protocol, please refer to Zhao et al.1

引用

页数：12

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