Developmental and Molecular Effects of C-Type Natriuretic Peptide Supplementation in In Vitro Culture of Bovine Embryos

被引:0
|
作者
Costa, Camila Bortoliero [1 ,2 ,3 ]
da Silva, Nathalia Covre [3 ]
Silva, Amanda Nespolo [4 ,5 ]
Pioltine, Elisa Mariano [2 ]
Dellaqua, Thaisy Tino [1 ]
Zangirolamo, Amanda Fonseca [3 ,6 ]
Meirelles, Flavio Vieira [5 ]
Seneda, Marcelo Marcondes [3 ,6 ]
Nogueira, Marcelo Fabio Gouveia [1 ,2 ]
机构
[1] Sao Paulo State Univ UNESP, Inst Biosci, Grad Program Pharmacol & Biotechnol, BR-18618970 Botucatu, SP, Brazil
[2] Sao Paulo State Univ UNESP, Sch Sci Humanities & Languages, Dept Biol Sci, BR-19806900 Assis, SP, Brazil
[3] Univ Londrina UEL, Dept Vet Med, Lab Anim Reprod, BR-86057970 Londrina, PR, Brazil
[4] Univ Sao Paulo, Grad Program Anat Domest & Wild Anim, BR-13635000 Pirassununga, SP, Brazil
[5] Univ Sao Paulo, Coll Anim Sci & Food Engn, Dept Vet Med, BR-13635000 Pirassununga, SP, Brazil
[6] Univ Londrina UEL, Natl Inst Sci & Technol Dairy Prod Chain INCT LEIT, BR-86057970 Londrina, PR, Brazil
基金
巴西圣保罗研究基金会;
关键词
C-type natriuretic peptide; embryonic metabolism; NPR2; transcript abundance; cattle; ENDOPLASMIC-RETICULUM; COMPETENCE; EXPRESSION; COMPLEXES; OOCYTES; GENE; IVM;
D O I
10.3390/ijms252010938
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The use of C-type natriuretic peptide (CNP) in the interaction with the oocyte and in the temporary postponement of spontaneous meiosis resumption has already been well described. However, its action in pre-implantation developmental-stage embryos is yet to be understood. Thus, our study aimed to detect the presence of the canonical CNP receptor (natriuretic peptide receptor, NPR2) in germinal vesicle (GV)-, metaphase II (MII)-, presumptive zygote (PZ)-, morula (MO)-, and blastocyst (BL)-stage embryos and, later, to observe possible modulations on the embryos when co-cultured with CNP. In Experiment I, we detected and quantified NPR2 on the abovementioned embryo stages. Further, in Experiment II, we intended to test different concentrations (100, 200, or 400 nM of CNP) at different times of inclusion in the in vitro culture (IVC; inclusion from the beginning, i.e., day 1, or from day 5). In Experiment III, 400 nM of CNP was used on day 1 (D1) in the IVC, which was not demonstrated to be embryotoxic, and it showed potentially promising results in the blastocyst production rate when compared to the control. Thus, we analyzed the embryonic development rates of bovine embryos (D7) and hatching kinetics (D7, D8, and D9). Subsequently, morula and blastocyst were collected and evaluated for transcript abundance of their competence and quality (apoptosis, oxidative stress, proliferation, and differentiation) and lipid metabolism. Differences with probabilities less than p < 0.05, and/or fold change (FC) > 1.5, were considered significant. We demonstrate the presence of NPR2 until the blastocyst development stage, when there was a significant decrease in membrane receptors. There was no statistical difference in the production rate after co-culture with 400 nM CNP. However, when we evaluated the abundance of morula transcripts, there was an upregulated transcription in ADCY6 (p = 0.057) and downregulated transcripts in BMP15 (p = 0.013), ACAT1 (p = 0.040), and CASP3 (p = 0.082). In addition, there was a total of 12 transcriptions in morula that presented variation FC > 1.5. In blastocysts, the treatment with CNP induced upregulation in BID, CASP3, SOX2, and HSPA5 transcripts and downregulation in BDNF, NLRP5, ELOVL1, ELOVL4, IGFBP4, and FDX1 transcripts (FC > 1.5). Thus, our study identified and quantified the presence of NPR2 in bovine pre-implantation embryos. Furthermore, 400 nM of CNP in IVC, a concentration not previously described in the literature, modulated some transcripts related to embryonic metabolism, and this was not embryotoxic morphologically.
引用
收藏
页数:15
相关论文
共 50 条
  • [21] Physiological and Pathophysiological Effects of C-Type Natriuretic Peptide on the Heart
    Yasoda, Akihiro
    BIOLOGY-BASEL, 2022, 11 (06):
  • [22] Effects of intravenously administered C-type natriuretic peptide in humans: Comparison with atrial natriuretic peptide
    Igaki, T
    Itoh, H
    Suga, S
    Hama, N
    Ogawa, Y
    Komatsu, Y
    Yamashita, J
    Doi, K
    Chun, T
    Nakao, K
    HYPERTENSION RESEARCH-CLINICAL AND EXPERIMENTAL, 1998, 21 (01): : 7 - 13
  • [23] Supplementation of c-type natriuretic peptide during in vitro growth period benefits the development of murine preantral follicles
    Li Ang
    Cao Haixia
    Li Hongxia
    Li Ruijiao
    Guo Xingping
    Wang Huaixiu
    ZYGOTE, 2021, 29 (02) : 150 - 154
  • [24] Atrial natriuretic peptide, brain natriuretic peptide and c-type natriuretic peptide: Effects on testicular microcirculation and immunohistochemical localization
    Collin, O
    Lissbrant, E
    Bergh, A
    INTERNATIONAL JOURNAL OF ANDROLOGY, 1997, 20 (01): : 55 - 60
  • [25] C-type natriuretic peptide: The endothelial component of the natriuretic peptide system
    Chen, HH
    Burnett, JC
    JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 1998, 32 : S22 - S28
  • [26] C-TYPE NATRIURETIC PEPTIDE - A SELECTIVE CARDIOVASCULAR PEPTIDE
    CLAVELL, AL
    STINGO, AJ
    WEI, CM
    HEUBLEIN, DM
    BURNETT, JC
    AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 264 (02): : R290 - R295
  • [27] Molecular Mechanism of Induction of Bone Growth by the C-Type Natriuretic Peptide
    Rintz, Estera
    Wegrzyn, Grzegorz
    Fujii, Toshihito
    Tomatsu, Shunji
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (11)
  • [28] Biphasic in vitro maturation with C-type natriuretic peptide enhances the developmental competence of juvenile-goat oocytes
    Soto-Heras, Sandra
    Menendez-Blanco, Irene
    Catala, Maria-Gracia
    Izquierdo, Dolors
    Thompson, Jeremy G.
    Paramio, Maria-Teresa
    PLOS ONE, 2019, 14 (08):
  • [29] The Effect of Culture Methods and Serum Supplementation on Developmental Competence of Bovine Embryos Cultured In vitro
    Jung, Song-yi
    Willard, Scott T.
    JOURNAL OF ANIMAL AND VETERINARY ADVANCES, 2009, 8 (01): : 67 - 74
  • [30] Pre-IVM treatment with C-type natriuretic peptide in the presence of cysteamine enhances bovine oocytes antioxidant defense ability and developmental competence in vitro
    Zhenwei, J.
    Xianhua, Z.
    IRANIAN JOURNAL OF VETERINARY RESEARCH, 2019, 20 (03) : 173 - 179