Background: This study examined the effectiveness of using 3D virtual anatomy simulation on the perceived learning achievement, satisfaction, and flow state of nursing students. Methods: In this quasi-experimental study conducted with undergraduate nursing students, the experimental group underwent a five-week 3D virtual anatomy simulation program, while both the experimental and control groups attended the same pathophysiology classes. Data were collected through surveys. Results: Perceived learning achievement (t = 4.25, p = .001), satisfaction (t = 3.87, p < .001), and flow state (t = 5.11, p < .001) were statistically significantly higher in the experimental group. Conclusions: 3D virtual cadaveric simulation may be effective for enhancing nursing students' perceived learning achievement, satisfaction, and flow state.