Generation of a homozygous CPAMD8 knockout human embryonic stem cell line (WAe009-A-2R) by CRISPR/Cas9 system

被引：0
|
作者：
Pan, Wenmin [1 ,2 ,3 ]
Yang, Yuhe [2 ]
Zhang, Shun [1 ,2 ,3 ]
Liu, Xiaoyu [2 ]
Hu, Huijuan [2 ]
Qu, Jia [1 ,2 ,3 ,4 ]
Liu, Hui [2 ,4 ]
机构：
[1] Hainan Univ, Sch Biomed Engn, Haikou, Hainan, Peoples R China
[2] Wenzhou Med Univ, Eye Hosp, Natl Engn Res Ctr Ophthalmol & Optometry, Wenzhou, Peoples R China
[3] Hainan Inst Real World Data, Qionghai, Peoples R China
[4] Zhejiang Lab Regenerat Med Vis & Brain Hlth, Oujiang Lab, Wenzhou, Peoples R China
来源：
STEM CELL RESEARCH | 2025年 / 84卷
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1016/j.scr.2025.103683
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
CPAMD8, a constituent of the A2M/C3 (alpha-2-macroglobulin/complement 3) protein family, is strikingly expressed in the human fetal lens and distal neural retina. Mutations in CPAMD8 have been linked to anterior segment dysgenesis and primary congenital glaucoma. We utilized CRISPR/Cas9 technology to establish a homozygous CPAMD8 knockout human embryonic stem cell line for differentiating retinal organoids, with the intent of exploring the role of CPAMD8 in the early development of the human eye. The CPAMD8 knockout cell line exhibits normal morphology, pluripotency, and karyotype, serving as a valuable research tool for investigating the functions of CPAMD8 in ophthalmology.
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页数：5
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