Innovative immobilization of (3-glucosidase on amidoximated acrylic fabric integrated with magnetite Fe3O4 nanoparticles

This research presents a novel system for immobilizing (3-glucosidase on amidoximated acrylic fabric integrated with magnetite nanoparticles. Amidoximated acrylic fabric (TAC) was prepared using hydroxylamine hydrochloride, and magnetite nanoparticles were incorporated to create TAC-Fe3O4, which was subsequently activated with glutaraldehyde and used to immobilize (3-glucosidase. The immobilization efficiency reached an 89 % yield. Characterization techniques such as FTIR, SEM, and BET analysis confirmed successful immobilization, whereas kinetic studies revealed an increase in Km (7.83 mM for TAC- Fe3O4@(3-Glu vs. 5.45 mM for free enzyme). The free (3-glucosidase exhibited maximum activity at pH 5.5, while TAC-Fe3O4@(3-Glu demonstrated an optimal pH of 6.0. Similarly, the free enzyme showed maximum activity at 40 degrees C, whereas the immobilized enzyme achieved its maximum activity at 50 degrees C. Operational stability tests revealed that TAC-Fe3O4@(3-glucosidase retained 79 % of its initial activity after 10 reuse cycles and 72 % after 15 cycles. Storage stability studies demonstrated a significant advantage for the immobilized enzyme, which maintained 85 % of its activity after 8 weeks at 4 degrees C, compared with 52 % for the free enzyme. These results highlight the potential of TAC-Fe3O4 as a cost-effective and scalable system for enzyme immobilization, offering improved stability and reusability, making it highly suitable for industrial applications.