YKL-40 is structurally similar to chitotriosidase (CHIT1), an active chitinase, but it lacks chitin-degrading activity while retaining chitin-binding capability. Elevated YKL-40 levels are associated with inflammatory diseases and cancers, making it a valuable biomarker. We previously reported that the W69T substitution in YKL-40 significantly reduces its chitin-binding affinity, identifying W69 as a crucial binding site. In this study, we establish a novel chitin-binding affinity evaluation method using a three-step buffer system to assess the binding strength and specificity of chitin-binding proteins and apply it to characterize YKL-40's binding mechanism. Our findings confirm that YKL-40, through its key residue W69, exhibits highly specific and robust affinity to chitin. Unlike CHIT1, which has both a catalytic domain (CatD) and a chitin-binding domain (CBD) that allow for diverse binding and degradation activities, YKL-40 lacks a CBD and is specialized for specific chitin recognition without degrading it. Comparative analysis with YKL-39, which does not contain a corresponding W69 residue, highlights the unique role of this residue in YKL-40's chitin-binding activity that is potentially linked to immune and inflammatory responses. Our evaluation method clarifies YKL-40's binding properties and provides a versatile approach applicable to other chitin-binding proteins.
机构:
Buddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Res, Taipei 23142, Taiwan
Chinese Culture Univ, Dept Life Sci, Taipei 11114, TaiwanBuddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Res, Taipei 23142, Taiwan
Wu, Semon
Hsu, Lung-An
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Chang Gung Mem Hosp, Dept Internal Med, Cardiovasc Div 1, Taoyuan 33305, Taiwan
Chang Gung Univ, Coll Med, Taoyuan 33305, TaiwanBuddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Res, Taipei 23142, Taiwan
Hsu, Lung-An
Cheng, Shih-Tsung
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Buddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Internal Med, Div Cardiol, Taipei 23142, Taiwan
Buddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Ctr Cardiovasc, Taipei 23142, TaiwanBuddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Res, Taipei 23142, Taiwan
Cheng, Shih-Tsung
Teng, Ming-Sheng
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Buddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Res, Taipei 23142, TaiwanBuddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Res, Taipei 23142, Taiwan
Teng, Ming-Sheng
Yeh, Ching-Hua
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Buddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Res, Taipei 23142, TaiwanBuddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Res, Taipei 23142, Taiwan
Yeh, Ching-Hua
Sun, Yu-Chen
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Chang Gung Mem Hosp, Dept Lab Med, Taipei 33305, TaiwanBuddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Res, Taipei 23142, Taiwan
Sun, Yu-Chen
Huang, Hsuan-Li
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Buddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Res, Taipei 23142, TaiwanBuddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Res, Taipei 23142, Taiwan
机构:
Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New DelhiDepartment of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi
Naglot S.
Dalal K.
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Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New DelhiDepartment of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi
Dalal K.
Aggarwal P.
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机构:
Department of Emergency Medicine, All India Institute of Medical Sciences, Ansari Nagar, New DelhiDepartment of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi
Aggarwal P.
Dada R.
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机构:
Department of Anatomy, All India Institute of Medical Sciences, Ansari Nagar, New DelhiDepartment of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi
机构:
Chinese Univ Hong Kong, Sch Biomed Sci, Room 705,Kwee Seong Integrated Biomed Sci Bldg, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Sch Biomed Sci, Room 705,Kwee Seong Integrated Biomed Sci Bldg, Hong Kong, Peoples R China
Wang, Yubing
Wong, Chi Wai
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Chinese Univ Hong Kong, Sch Biomed Sci, Room 705,Kwee Seong Integrated Biomed Sci Bldg, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Sch Biomed Sci, Room 705,Kwee Seong Integrated Biomed Sci Bldg, Hong Kong, Peoples R China
Wong, Chi Wai
Yan, Mingfei
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Chinese Univ Hong Kong, Sch Biomed Sci, Room 705,Kwee Seong Integrated Biomed Sci Bldg, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Sch Biomed Sci, Room 705,Kwee Seong Integrated Biomed Sci Bldg, Hong Kong, Peoples R China
Yan, Mingfei
Li, Lisha
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Chinese Univ Hong Kong, Sch Biomed Sci, Room 705,Kwee Seong Integrated Biomed Sci Bldg, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Sch Biomed Sci, Room 705,Kwee Seong Integrated Biomed Sci Bldg, Hong Kong, Peoples R China
Li, Lisha
Liu, Tian
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Chinese Univ Hong Kong, Sch Biomed Sci, Room 705,Kwee Seong Integrated Biomed Sci Bldg, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Sch Biomed Sci, Room 705,Kwee Seong Integrated Biomed Sci Bldg, Hong Kong, Peoples R China
Liu, Tian
Or, Penelope Mei-Yu
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Chinese Univ Hong Kong, Sch Biomed Sci, Room 705,Kwee Seong Integrated Biomed Sci Bldg, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Sch Biomed Sci, Room 705,Kwee Seong Integrated Biomed Sci Bldg, Hong Kong, Peoples R China
Or, Penelope Mei-Yu
Tsui, Stephen Kwok-Wing
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Chinese Univ Hong Kong, Sch Biomed Sci, Room 705,Kwee Seong Integrated Biomed Sci Bldg, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Sch Biomed Sci, Room 705,Kwee Seong Integrated Biomed Sci Bldg, Hong Kong, Peoples R China
Tsui, Stephen Kwok-Wing
Waye, Mary Miu-Yee
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机构:
Chinese Univ Hong Kong, Nethersole Sch Nursing, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Sch Biomed Sci, Room 705,Kwee Seong Integrated Biomed Sci Bldg, Hong Kong, Peoples R China
Waye, Mary Miu-Yee
Chan, Andrew Man-Lok
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Chinese Univ Hong Kong, Sch Biomed Sci, Room 705,Kwee Seong Integrated Biomed Sci Bldg, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Sch Biomed Sci, Room 705,Kwee Seong Integrated Biomed Sci Bldg, Hong Kong, Peoples R China
机构:
Univ Malaya, Fac Pharm, Dept Clin Pharm, Kuala Lumpur 50603, MalaysiaUniv Malaya, Fac Pharm, Dept Clin Pharm, Kuala Lumpur 50603, Malaysia
Perumalsamy, Sangeetha
Huri, Hasniza Zaman
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Univ Malaya, Fac Pharm, Dept Clin Pharm, Kuala Lumpur 50603, Malaysia
Univ Malaya, Clin Invest Ctr, Med Ctr, Petaling Jaya 59100, MalaysiaUniv Malaya, Fac Pharm, Dept Clin Pharm, Kuala Lumpur 50603, Malaysia
Huri, Hasniza Zaman
Ahmad, Wan Azman Wan
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Univ Malaya, Fac Med, Cardiol Unit, Dept Med, Kuala Lumpur 50603, MalaysiaUniv Malaya, Fac Pharm, Dept Clin Pharm, Kuala Lumpur 50603, Malaysia
Ahmad, Wan Azman Wan
METABOLISM-CLINICAL AND EXPERIMENTAL,
2021,
116
: 22
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22
机构:
Buddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Internal Med, Div Cardiol, 289 Jianguo Rd, New Taipei 23142, Taiwan
Tzu Chi Univ, Sch Med, Hualien, TaiwanBuddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Internal Med, Div Cardiol, 289 Jianguo Rd, New Taipei 23142, Taiwan
Chou, Hsin-Hua
Teng, Ming-Sheng
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Buddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Res, New Taipei, TaiwanBuddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Internal Med, Div Cardiol, 289 Jianguo Rd, New Taipei 23142, Taiwan
Teng, Ming-Sheng
Juang, Jyh-Ming Jimmy
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机构:
Natl Taiwan Univ Hosp, Cardiovasc Ctr, Taipei, Taiwan
Natl Taiwan Univ Hosp, Dept Internal Med, Div Cardiol, Taipei, Taiwan
Natl Taiwan Univ, Coll Med, Taipei, TaiwanBuddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Internal Med, Div Cardiol, 289 Jianguo Rd, New Taipei 23142, Taiwan
Juang, Jyh-Ming Jimmy
Chiang, Fu-Tien
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Natl Taiwan Univ Hosp, Cardiovasc Ctr, Taipei, Taiwan
Natl Taiwan Univ Hosp, Dept Internal Med, Div Cardiol, Taipei, Taiwan
Natl Taiwan Univ, Coll Med, Taipei, Taiwan
Fu Jen Catholic Univ Hosp, Cardiovasc Ctr, Div Cardiol, New Taipei, TaiwanBuddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Internal Med, Div Cardiol, 289 Jianguo Rd, New Taipei 23142, Taiwan
Chiang, Fu-Tien
Tzeng, I-Shiang
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机构:
Buddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Res, New Taipei, TaiwanBuddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Internal Med, Div Cardiol, 289 Jianguo Rd, New Taipei 23142, Taiwan
Tzeng, I-Shiang
Wu, Semon
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机构:
Buddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Res, New Taipei, Taiwan
Chinese Culture Univ, Dept Life Sci, Taipei, TaiwanBuddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Internal Med, Div Cardiol, 289 Jianguo Rd, New Taipei 23142, Taiwan