PRODUCTION AND CRYOPRESERVATION OF 3D CULTURES

被引：0
|
作者：
Moisieieva, Nataliia [1 ]
Gorina, Olga [1 ]
Moisieiev, Anton [1 ]
Prokopiuk, Olga [1 ]
机构：
[1] Natl Acad Sci Ukraine, Inst Problems Cryobiol & Cryomed, 23,Pereyaslavska Str, UA-61016 Kharkiv, Ukraine
来源：
CRYOLETTERS | 2025年 / 46卷 / 01期
关键词：
cryopreservation; spheroid; parameters affecting the spheroid formation; theoretical; TUMOR SPHEROID FORMATION; IN-VITRO; CELL SPHEROIDS; HEPATOCYTE SPHEROIDS; DIMETHYL-SULFOXIDE; MASS-TRANSFER; MODEL; MEMBRANE; STRESS; ORGANOIDS;
D O I：
10.54680/fr25110110112
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Three-dimensional (3D) culture systems, which include spheroids (SPs), provide a unique platform for studying complex biological processes in vivo and for enhancing the capabilities of in vitro test systems. Their uniqueness lies in the 3D organization of cells and in the reproduction of complex intercellular interactions, similar to those in native tissues and organs. These "mini-organs" can be used for fundamental research, tissue-engineering constructs, development of preclinical models for testing pharmacological drugs, etc. Important and current issues regarding SPs involve improving methods for their production and cryopreservation. Solving these issues will expand the range and on factors influencing the formation of SPs, which can enhance the understanding of their correct
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页码：1 / 13
页数：13
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