In this study, the ecological pressure intensity-ecological sensitivity-ecological resilience (PSR) model was used to build a comprehensive index of ecological and environmental vulnerability to analyze the characteristics of ecological and environmental vulnerability in Gansu Province of China, and the influence degree of spatial and temporal distribution difference of ecological, environmental vulnerability in Gansu Province of China during 1992-2022 was analyzed. The results showed that (1) the eco-environmental vulnerability index of Gansu Province of China varied from 1.4857 to 4.0896, presenting the grades of mild vulnerability. Regarding spatial distribution, Longnan city and Tianshui city in Gansu Province of China have higher vulnerability levels, while Jiuquan city, Jiayuguan city, Zhangye city and Jinchang city have lower vulnerability levels. (2) Among the land use types in Gansu Province of China, cultivated land was mainly distributed in the mildly vulnerable and moderately vulnerable areas, forest land was mainly distributed in the mildly vulnerable, grassland was mainly distributed in the mildly vulnerable areas, water and construction land were distributed in the potentially vulnerable areas. (3) Average annual temperature, average annual precipitation, biological richness index were the main factors affecting the spatial distribution of eco-environmental vulnerability in Gansu Province of China.