Generation of human induced pluripotent stem cell lines (iPSC) from adipose-derived mesenchymal stromal cells from two patients with systemic sclerosis

被引：0

作者：

Velier, Melanie ^{[1
,2
]}

Appay, Romain ^{[3
,4
]}

Arcani, Robin ^{[1
,5
,6
]}

Desprat, Romain ^{[7
]}

Simoncini, Stephanie ^{[1
]}

Zavarro, Anouck ^{[1
]}

Noel, Daniele ^{[8
]}

Bendaass, Lenha ^{[1
]}

Jorgensen, Christian ^{[7
,8
,10
]}

De Pinho, Quentin Gomes ^{[1
,9
]}

Benyamine, Audrey ^{[1
,9
]}

Granel, Brigitte ^{[1
,9
]}

Daumas, Aurelie ^{[1
,5
,6
]}

George, Francoise Dignat ^{[1
]}

Sabatier, Florence ^{[1
,2
]}

Magalon, Jeremy ^{[1
,2
]}

机构：

[1] Aix Marseille Univ, INSERM 1263, INRAE1260, C2VN, Marseille, France

[2] Hop Conception, AP HM, INSERM, CIC BT 1409,Culture & Cell Therapy Lab, Marseille, France

[3] Hop La Timone, AP HM, Dept Pathol Anat & Neuropathol, Marseille, France

[4] Aix Marseille Univ, CNRS, Inst Neurophysiopathol, Marseille, France

[5] Hop La Timone, AP HM, Dept Internal Med, Marseille, France

[6] Hop La Timone, AP HM, Therapeut Dept, Marseille, France

[7] CHU Montpellier, Plateforme SAFE iPS, IRMB, Montpellier, France

[8] Univ Montpellier, INSERM, IRMB, Montpellier, France

[9] Hop Nord Marseille, AP HM, Internal Med Dept, Marseille, France

[10] CHU Montpellier, Clin Immunol & Osteoarticular Dis Therapeut Unit, Dept Rheumatol, Montpellier, France

来源：

STEM CELL RESEARCH | 2025年 / 82卷

关键词：

D O I：

10.1016/j.scr.2024.103624

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Systemic sclerosis (SSc) is a rare and complex connective tissue disease associated with high morbidity and mortality. SSc is characterized by ischemic vasculopathy, cutaneous and visceral fibrosis and a dysimmune state (Denton and Khanna, 2017; Volkmann et al., 2023; Barnes and Mayes, 2012). We have derived induced pluripotent stem cell (iPSC) lines from two SSc patients aged 38 and 67 years with severe vascular phenotype. These iPSC lines expressed pluripotent markers, exhibited normal and stable genome, and differentiated into trilineage embryonic layers in teratoma formation assays. These SSc-specific iPSC lines can be differentiated into endothelial cells, providing a valuable model to elucidate vascular dysfunction and develop personalized therapeutic approaches.

引用

页数：4

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