LcMYB5, an R2R3-MYB family gene from Lonicera caerulea L., enhances drought and salt tolerance in transgenic tobacco and blue honeysuckle

被引:0
|
作者
Fu, Chunlin [1 ,2 ]
Bian, Chunyang [1 ,2 ]
Chen, Jing [1 ,2 ]
Zhang, Qian [1 ,2 ]
Qin, Dong [1 ,2 ]
Li, Jiangkuo [3 ,4 ]
Zhang, Peng [3 ,4 ]
Huo, Junwei [1 ,2 ]
Gang, Huixin [1 ,2 ]
机构
[1] Northeast Agr Univ, Coll Hort & Landscape Architecture, Key Lab Biol & Genet Improvement Hort Crops Northe, Minist Agr & Rural Affairs, Harbin 150030, Peoples R China
[2] Northeast Agr Univ, Natl Local Joint Engn Res Ctr Dev & Utilizat Small, Harbin 150030, Peoples R China
[3] Tianjin Acad Agr Sci, Inst Agr Prod Preservat & Proc Technol, Tianjin 300384, Peoples R China
[4] Natl Engn & Technol Res Ctr Preservat Agr Prod, Tianjin Key Lab Postharvest Physiol & Storage Agr, Tianjin 300384, Peoples R China
基金
中国博士后科学基金;
关键词
Drought stress; LcMYB5; Lonicera caerulea L; Salt stress; Tobacco; MYB TRANSCRIPTION FACTOR; STRESS TOLERANCE; SIGNAL-TRANSDUCTION; CONFERS TOLERANCE; OVER-EXPRESSION; CHLOROPHYLL-A; COLD; ANTHOCYANIN; RESPONSES; ENCODES;
D O I
10.1016/j.jplph.2024.154409
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
MYB transcription factors exert crucial functions in enhancing plant stress tolerance, which is impacted by soil drought and salinity. In our study, the R2R3-type MYB transcription factor gene LcMYB5 from blue honeysuckle ( Lonicera caerulea L.) was successfully cloned and identified, and confirmed its nuclear localization. LcMYB5 overexpression was vastly enhanced drought and salt tolerance in both blue honeysuckle and tobacco seedlings. After drought stress, transgenic tobacco exhibited an average survival rate of 70.30%, while most wild-type (WT) plants perished, resulting in a survival rate of only 15.33%. Following salt stress, the average survival rate for transgenic tobacco reached 77.24%, compared to just 22.47% for WT plants. Measurements indicated, that transgenic tobacco had higher proline content than WT, as well as higher superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activity. Transgenic tobacco decreased chlorophyll content less dramatically than WT tobacco, despite both tobaccos having decreased chlorophyll content. Furthermore, the level of malondialdehyde (MDA) and relative conductivity were lower in transgenic tobacco compared to WT. Furthermore, LcMYB5 overexpression significantly increased the expression levels of key genes related to drought stress ( NCED1 , NCED2, PYL4, PYL8, and CBL1) and salt stress ( NHX1 , SOD, CAT1, SOS1, and HSP17.8), thus improving transgenic tobacco's stress tolerance. Compared to WT blue honeysuckle, transiently transformed LcMYB5-expressing blue honeysuckle exhibited milder damage under stress conditions, a significant increase in chlorophyll and proline content was observed, the activities of SOD, POD and CAT were also significantly increased, the increase in MDA content and relative conductivity is relatively small. Additionally, In addition, transient expression of LcMYB5 can also positively regulate the expression of these five key genes of drought stress and five key genes of salt stress, so as to improve the resistance of transgenic blue honeysuckle to drought and salt stress. In summary, our study reveals the important regulatory role of LcMYB5 in plant resistance to drought and salt stress, providing theoretical support and potential application value for further improving crop stress resistance.
引用
收藏
页数:12
相关论文
共 50 条
  • [41] Genome-Wide Identification and Capsaicinoid Biosynthesis-Related Expression Analysis of the R2R3-MYB Gene Family in Capsicum annuum L.
    Wang, Jin
    Liu, Yi
    Tang, Bingqian
    Dai, Xiongze
    Xie, Lingling
    Liu, Feng
    Zou, Xuexiao
    FRONTIERS IN GENETICS, 2020, 11
  • [42] Genome-wide study of the R2R3-MYB gene family and analysis of HhMYB111r-induced salt tolerance in Hibiscus hamabo Sieb. et Zucc
    Xu, Yu
    Ni, Longjie
    Yu, Chaoguang
    Hua, Jianfeng
    Yin, Yunlong
    Gu, Chunsun
    Wang, Zhiquan
    PLANT SCIENCE, 2025, 352
  • [43] Overexpression of a R2R3 MYB gene MdSIMYB1 increases tolerance to multiple stresses in transgenic tobacco and apples
    Wang, Rong-Kai
    Cao, Zhong-Hui
    Hao, Yu-Jin
    PHYSIOLOGIA PLANTARUM, 2014, 150 (01) : 76 - 87
  • [44] Overexpression of the Wild Soybean R2R3-MYB Transcription Factor GsMYB15 Enhances Resistance to Salt Stress and Helicoverpa Armigera in Transgenic Arabidopsis
    Shen, Xin-Jie
    Wang, Yan-Yan
    Zhang, Yong-Xing
    Guo, Wei
    Jiao, Yong-Qing
    Zhou, Xin-An
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2018, 19 (12)
  • [45] A R2R3-MYB Transcription Factor Gene, BpMYB123, Regulates BpLEA14 to Improve Drought Tolerance in Betula platyphylla
    Lv, Kaiwen
    Wei, Hairong
    Liu, Guifeng
    FRONTIERS IN PLANT SCIENCE, 2021, 12
  • [46] Ectopic expression of the R2R3-MYB gene from Tricyrtis sp results in leaf color alteration in transgenic Pelargonium crispum
    Kanemaki, Atsushi
    Otani, Masahiro
    Takano, Miho
    Fujimoto, Takuo
    Okuhara, Hiroaki
    Nomizu, Toshikazu
    Kondo, Masayoshi
    Kobayashi, Hitoshi
    Tatsuzawa, Fumi
    Nakano, Masaru
    SCIENTIA HORTICULTURAE, 2018, 240 : 411 - 416
  • [47] VcMYB4a, an R2R3-MYB transcription factor from Vaccinium corymbosum, negatively regulates salt, drought, and temperature stress
    Zhang, Chun-Yu
    Liu, Hong-Chao
    Zhang, Xin-Sheng
    Guo, Qing-Xun
    Bian, Shao-Min
    Wang, Jing-Ying
    Zhai, Lu-Lu
    GENE, 2020, 757
  • [48] Identification of a MYB3R gene involved in drought, salt and cold stress in wheat (Triticum aestivum L.)
    Cai, Hongsheng
    Tian, Shan
    Liu, Changlai
    Dong, Hansong
    GENE, 2011, 485 (02) : 146 - 152
  • [49] Tartary buckwheat FtMYB10 encodes an R2R3-MYB transcription factor that acts as a novel negative regulator of salt and drought response in transgenic Arabidopsis
    Gao, Fei
    Yao, Huipeng
    Zhao, Haixia
    Zhou, Jing
    Luo, Xiaopeng
    Huang, Yunji
    Li, Chenglei
    Chen, Hui
    Wu, Qi
    PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2016, 109 : 387 - 396
  • [50] R2R3-MYB Transcription Factor NtMYB330 Regulates Proanthocyanidin Biosynthesis and Seed Germination in Tobacco (Nicotiana tabacum L.)
    Zhao, Lu
    Song, Zhongbang
    Wang, Bingwu
    Gao, Yulong
    Shi, Junli
    Sui, Xueyi
    Chen, Xuejun
    Zhang, Yihan
    Li, Yongping
    FRONTIERS IN PLANT SCIENCE, 2022, 13