Image denoising based on two widespread algorithms in femtosecond stimulated Raman scattering microscopy

被引：0

作者：

Costa, Giovanni ^{[1
,2
]}

Ranjan, Rajeev ^{[1
,3
]}

Ferrara, Maria Antonietta ^{[1
]}

Sansone, Mario ^{[4
]}

Sirleto, Luigi ^{[1
]}

机构：

[1] Natl Res Council CNR, Inst Appl Sci & Intelligent Syst, Naples, Italy

[2] Eindhoven Univ Technol TU E, Dept Elect Engn, Eindhoven, Netherlands

[3] Aix Marseille Univ, Inst Fresnel, Cent Marseille, CNRS, F-13013 Marseille, France

[4] Univ Naples Federico II, Dept Elect Engn & Informat Technol DIETI, Naples, Italy

来源：

2024 24TH INTERNATIONAL CONFERENCE ON TRANSPARENT OPTICAL NETWORKS, ICTON 2024 | 2024年

关键词：

nonlinear microscopy; label-free imaging; stimulated Raman scattering; noise; image denoising;

D O I：

10.1109/ICTON62926.2024.10648127

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Stimulated Raman scattering (SRS) microscopy is able to perform high sensitivity biological imaging with high spatial and spectral resolution and image acquisition time of a few seconds. Nevertheless, SRS images often suffer from low SNR, due to the weak Raman cross-section of biomolecules. Therefore, methods aiming to improve image quality are mandatory. In this paper, the performances of two widespread general algorithms, i.e. averaging and Karhunen-Loeve Transform (KLT) are compared. SRS imaging of lipids droplets have been used in order to validate our algorithms.

引用

页数：4

共 50 条

[31] Two Dimensional Femtosecond Stimulated Raman Spectroscopy
Wilson, Kristina C.
Mehlenbacher, Randy
Lyons, Brendon
McCamant, David W.
XXII INTERNATIONAL CONFERENCE ON RAMAN SPECTROSCOPY, 2010, 1267 : 537 - 538
[32] Photoswitchable stimulated Raman scattering spectroscopy and microscopy
Shou, Jingwen
Ozeki, Yasuyuki
OPTICS LETTERS, 2021, 46 (09) : 2176 - 2179
[33] Chirp modulation stimulated Raman scattering microscopy
Pegoraro, Adrian F.
Stolow, Albert
OPTICS EXPRESS, 2024, 32 (18): : 31297 - 31310
[34] Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
De la Cadena, Alejandro
Vernuccio, Federico
Talone, Benedetta
Bresci, Arianna
Ceconello, Chiara
Das, Subir
Vanna, Renzo
Cerullo, Giulio
Polli, Dario
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2022, (185):
[35] Subcellular chemical and morphological analysis by stimulated Raman scattering microscopy and image analysis techniques
D'Arco, Annalisa
Brancati, Nadia
Ferrara, Maria Antonietta
Indolfi, Maurizio
Frucci, Maria
Sirleto, Luigi
BIOMEDICAL OPTICS EXPRESS, 2016, 7 (05): : 1853 - 1864
[36] Simultaneous Dual-Band Hyperspectral Stimulated Raman Scattering Microscopy with Femtosecond Optical Parametric Oscillators
Xu, Fiona Xi
Rathbone, Emily G.
Fu, Dan
JOURNAL OF PHYSICAL CHEMISTRY B, 2023, 127 (10): : 2187 - 2197
[37] Label-free imaging of small lipid droplets by femtosecond-stimulated Raman scattering microscopy
D'Arco, Annalisa
Ferrara, Maria Antonietta
Indolfi, Maurizio
Tufano, Vitaliano
Sirleto, Luigi
JOURNAL OF NONLINEAR OPTICAL PHYSICS & MATERIALS, 2017, 26 (04)
[38] Femtosecond Stimulated Raman Spectroscopy and preliminary steps for nonlinear microscopy
D'Arco, A.
Indolfi, M.
Ferrara, M. A.
Brancati, N.
Zeni, L.
Sirleto, L.
2015 INTERNATIONAL CONFERENCE ON BIOPHOTONICS (BIOPHOTONICS), 2015, : 1 - 3
[39] Microfiber analysis via femtosecond stimulated Raman microscopy (FSRM)
Carolin Borbeck
Francisco van Riel Neto
Roman Bernst
Peter Gilch
Microplastics and Nanoplastics, 5 (1):
[40] Optimized amplitude mode modulation in femtosecond stimulated Raman microscopy
Lipkin, Maxim
Nixdorf, Jakob
Gilch, Peter
OPTICS LETTERS, 2020, 45 (15) : 4204 - 4207

← 1 2 3 4 5 →