The molecular mechanism underlying in vitro wound healing property of Stryphnodendron astringent (Mart.)

被引:0
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作者
Pellenz, Neida Luiza Kaspary [1 ]
Mastella, Moises Henrique [2 ]
Azzolin, Veronica Farina [1 ,3 ]
Teixeira, Cibele Ferreira [1 ]
Carpilovsky, Paula Kohler [4 ]
Brunelli, Lucas Muller [1 ]
Carpilovsky, Cristiane Kohler [1 ]
Costabeber, Ijoni Hilda [1 ]
da Cruz, Ivana Beatrice Manica [1 ]
Duarte, Marta Maria Medeiros Frescura [5 ]
Barbisan, Fernanda [1 ]
机构
[1] Univ Fed Santa Maria UFSM, Santa Maria, RS, Brazil
[2] Univ Estadual Paulista UNESP, Botucatu, SP, Brazil
[3] Fundacao Univ Aberta da Terceira Idade FuNATI, Manaus, AM, Brazil
[4] Univ Franciscana UFN, Santa Maria, RS, Brazil
[5] Univ Luterana Brasil ULBRA, Torres, RS, Brazil
关键词
polyphenols; wound healing; antioxidant; anti-inflammatory; FIBROBLASTS; KERATINOCYTES; BARBATIMAO; EXTRACTS;
D O I
10.15448/1980-6108.2025.1.46171
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Aim: to analyze the mechanism of the hydroalcoholic extract of barbatim & atilde;o in the induction of healing in dermalfibroblast cells (HFF-1), in an in vitro model. Methods: two concentrations of barbatim & atilde;o used in commercial ointments were studied here (B1 = 0.49 mg/mL; B2 = 0.99 mg/mL), and their effects were evaluated with the following markers at 24 and 72 hours after induction of the in vitro wound: specialIntscript oxidative metabolism [DNA oxidation, lipoperoxidation, protein carbonylation, antioxidant enzymes (superoxide dis- mutase, SOD; catalase, CAT; glutathione peroxidase, GPX)]; specialIntscript pro-inflammatory cytokines (TNF-, interleukin 6); specialIntscript apoptotic markers [caspase 3 (CASP3), CASP8, and growth factors: keratinocyte growth factor (KGF) and fibroblast growth factor-1 (FGF-1)]. Results: treatment with both concentrations showed a decrease in pro-oxidant, pro-inflammatory, and pro--apoptotic markers, while increasing antioxidant levels and growth factors. Conclusions: barbatim & atilde;o acts through a pleiotropic cascade mechanism involving antioxidant, anti-in- flammatory, anti-apoptotic, and fibroblast migration/ proliferation modulation. Our data could explain the effect and importance of barbatim & atilde;o extract in wound healing mechanisms.
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页数:12
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