Development of a rapid and ultrasensitive acrylamide nanosensor based on TiO2 NPs/GQDs nanocomposite

Acrylamide (AA), a well-known human carcinogen, is a toxic compound formed in thermally processed foods via the Millard reaction. This study summarizes a nanosensor-based rapid detection of AA. Pencil graphite (PG) electrode modified with Titanium dioxide nanoparticles (TiO2 NPs)/ Graphene Quantum Dots (GQDs) nano- composite and Hemoglobin (Hb) have been prepared for application as stable and sensitive AA nanosensor. The synthesized nanoparticles were characterized by Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), UV-visible spectroscopy, Transmission electron microscopy (TEM), X-ray diffraction analysis (XRD), and Zeta potential analysis. Cyclic voltammetry (CV), FTIR, and electrochemical Impedance (EIS) measurements were performed to characterize the working electrode at every modification stage. The optimum response was observed at 30 degrees C in phosphate buffer (pH 7.0). The developed nanosensor showed a wide linear range of 10 nM to 200 nM with a very low detection limit of 0.67 nM, low quantification limit (LOQ) of 2.23 nM, and a very high sensitivity of 2.40 mAcm- 2nM-1. The suggested strategy used less expensive materials to create a compelling and environmentally sustainable detection process. With the use of this innovative nanocomposite material, biosensing platforms for the on-site, ultrasensitive, and selective detection of different harmful chemicals in food safety studies can be developed.