Genetic Differences Modify Anesthetic Preconditioning of Traumatic Brain Injury in Drosophila

被引:0
|
作者
Johnson-Schlitz, Dena [1 ]
Seidl, Amanda R. [1 ]
Olufs, Zachariah P. G. [1 ]
Huang, Wen [2 ]
Wassarman, David A. [3 ]
Perouansky, Misha [1 ]
机构
[1] Univ Wisconsin, Sch Med & Publ Hlth, Dept Anesthesiol, Madison, WI 53706 USA
[2] Michigan State Univ, Dept Anim Sci, E Lansing, MI USA
[3] Univ Wisconsin, Sch Med & Publ Hlth, Dept Med Genet, Room 4262,Genet Biotechnol Bldg,425-G Henry Mall, Madison, WI 53706 USA
关键词
anesthetics; <italic>Drosophila melanogaster</italic>; genome-wide association study; isoflurane; sevoflurane; traumatic brain injury; ISOFLURANE ANESTHESIA; INTRACRANIAL-PRESSURE; ANIMAL-MODELS; MELANOGASTER; SEVOFLURANE; EXPRESSION; CHANNELS; NEUROPROTECTION; NEUROTOXICITY; ACTIVATION;
D O I
10.1089/neu.2024.0314
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Pre-clinical vertebrate models of traumatic brain injury (TBI) routinely use anesthetics for animal welfare; however, humans experience TBI without anesthetics. Therefore, translation of findings from vertebrate models to humans hinges on understanding how anesthetics influence cellular and molecular events that lead to secondary injuries following TBI. To investigate the effects of anesthetics on TBI outcomes, we used an invertebrate Drosophila melanogaster model to compare outcomes between animals exposed or not exposed to anesthetics prior to the same primary injury. Using a common laboratory fly line, w1118, we found that exposure to the volatile anesthetics isoflurane or sevoflurane, but not ether, prior to TBI produced a dose-dependent reduction in mortality within 24 h following TBI. Thus, isoflurane and sevoflurane precondition w1118 flies to deleterious effects of TBI. To examine the effects of genetic differences on anesthetic preconditioning of TBI, we repeated the experiment with the Drosophila Genetic Reference Panel (DGRP) collection of genetically diverse, inbred fly lines. Pre-exposure to either isoflurane or sevoflurane revealed a wide range of preconditioning levels among 171 and 144 DGRP lines, respectively, suggesting a genetic component for variation in anesthetic preconditioning of mortality following TBI. Finally, genome-wide association study analyses identified single-nucleotide polymorphisms in genes associated with isoflurane or sevoflurane preconditioning of TBI. Several of the genes, including the fly ortholog of mammalian Calcium Voltage-Gated Subunit Alpha1 D (CACNA1D), are highly expressed in neurons and are functionally linked to both anesthetics and TBI. These data indicate that anesthetic dose and genetic background should be considered when investigating effects of anesthetics in vertebrate TBI models, and they support use of the fly model for elucidating the mechanisms underlying anesthetic preconditioning of TBI.
引用
收藏
页数:13
相关论文
共 50 条
  • [31] Hyperbaric oxygen preconditioning protects against traumatic brain injury at high altitude
    Hu, S. L.
    Hu, R.
    Li, F.
    Liu, Z.
    Xia, Y. Z.
    Cui, G. Y.
    Feng, H.
    CEREBRAL HEMORRHAGE, 2008, 105 : 191 - 196
  • [32] Lipopolysaccharide preconditioning reduces the effect of traumatic brain injury on pentylenetetrazole kindled rats
    Eslami, M.
    Sayyah, M.
    Hajghassem, M.
    Solaymani, M.
    INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, 2015, 47 : 24 - 25
  • [33] REMOTE-ISCHEMIC PRECONDITIONING AS A PROPHYLACTIC TREATMENT FOR MILD TRAUMATIC BRAIN INJURY
    Park, Eugene
    Lalani, Misbah Nadeem
    Park, Katya J.
    Baker, Andrew J.
    JOURNAL OF NEUROTRAUMA, 2014, 31 (05) : A49 - A50
  • [34] Lipopolysaccharide preconditioning prevents acceleration of kindling epileptogenesis induced by traumatic brain injury
    Eslami, Mansoureh
    Sayyah, Mohammad
    Soleimani, Mansoureh
    Alizadeh, Leila
    Hadjighassem, Mahmoudreza
    JOURNAL OF NEUROIMMUNOLOGY, 2015, 289 : 143 - 151
  • [35] REMOTE ISCHEMIC PRECONDITIONING (RIPREC) PROTECTS FROM TRAUMATIC BRAIN INJURY (TBI)
    Vaibhav, K.
    Baban, B.
    Khan, M. B.
    Liu, J. Y.
    Huo, Y.
    Hess, D. C.
    Dhandapani, K. M.
    Hoda, M. N.
    JOURNAL OF NEUROTRAUMA, 2014, 31 (12) : A87 - A87
  • [36] Genetic Influences on Outcome Following Traumatic Brain Injury
    Barry D. Jordan
    Neurochemical Research, 2007, 32 : 905 - 915
  • [37] Genetic influences on outcome following traumatic brain injury
    Jordan, Barry D.
    NEUROCHEMICAL RESEARCH, 2007, 32 (4-5) : 905 - 915
  • [38] Genetic association studies in patients with traumatic brain injury
    Dardiotis, Efthimios
    Fountas, Kostas N.
    Dardioti, Maria
    Xiromerisiou, Georgia
    Kapsalaki, Eftychia
    Tasiou, Anastasia
    Hadjigeorgiou, Georgios M.
    NEUROSURGICAL FOCUS, 2010, 28 (01) : E9.1 - E9.12
  • [39] Anesthetic management of the traumatic brain injury patients undergoing non-neurosurgery
    Kim, Hyunjee
    ANESTHESIA AND PAIN MEDICINE, 2023, 18 (02): : 104 - 113
  • [40] Sleep loss, caffeine, sleep aids and sedation modify brain abnormalities of mild traumatic brain injury
    Everson, Carol A.
    Szabo, Aniko
    Plyer, Cade
    Hammeke, Thomas A.
    Stemper, Brian D.
    Budde, Mathew D.
    EXPERIMENTAL NEUROLOGY, 2024, 372