Detection of Gram-positive and Gram-negative bacteria in brain abscesses by 16S rRNA in situ hybridization

被引:0
|
作者
Mbongo, William [1 ]
Laga, Alvaro C. [1 ]
Solomon, Isaac H. [1 ]
机构
[1] Harvard Med Sch, Brigham & Womens Hosp, Dept Pathol, Boston, MA USA
来源
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY | 2024年 / 84卷 / 02期
基金
美国国家卫生研究院;
关键词
16S rRNA; brain abscess; Gram stain; infectious disease; in situ hybridization; molecular diagnostics; POLYMERASE-CHAIN-REACTION; IDENTIFICATION; DIAGNOSIS;
D O I
10.1093/jnen/nlae118
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
In situ hybridization (ISH) staining of bacterial 16S ribosomal RNA (rRNA) is an alternative to standard histological stains (eg, Gram, Warthin-Starry), and may improve the diagnosis of bacterial brain abscesses. To evaluate the utility of 16S rRNA ISH, a 10-year retrospective cohort was assembled from a large academic medical center. Results of histological stains, cultures, and 16S rRNA sequencing were extracted from reports, and new Gram and 16S rRNA ISH stains were performed. Histologically identifiable bacteria were present in 40/63 (63%) cases and 38/57 (67%) were associated with positive cultures. Overall, 16S rRNA ISH was positive in 18/63 (29%) cases, including 16/37 (43%) with positive Gram stains, 12/38 (32%) positive by culture, and 4/8 (50%) positive by sequencing. 16S rRNA ISH highlighted bacteria in 14/40 (35%) cases with Gram-positive organisms and 9/17 (53%) with Gram-negative organisms (including 6 polymicrobial cases). Compared to a composite gold standard of Gram stain and culture, the sensitivity and specificity of 16S rRNA ISH were 35% and 93%, respectively. While sensitivity is relatively low, 16S rRNA ISH may be useful for distinguishing real organisms from artifacts and for identifying brain abscess cases suitable for 16S rRNA sequencing.
引用
收藏
页码:141 / 146
页数:6
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