Development of Microsatellite Markers from Transcriptome of Eriocheir sinensis and Their Application in Multiplex PCR Panels

被引:0
|
作者
Xiao, Qizhen [1 ,2 ]
Tang, Meijun [1 ,3 ]
Li, Qingqing [1 ,4 ,5 ]
Dong, Pengsheng [1 ,4 ,5 ]
Cheng, Yongxu [1 ,4 ,5 ]
Deng, Deng [6 ]
Wu, Xugan [1 ,3 ,4 ]
机构
[1] Shanghai Ocean Univ, Key Lab Freshwater Aquat Genet Resources, Minist Agr, Shanghai 201306, Peoples R China
[2] Fujian Polytech Normal Univ, Fujian Prov Key Lab Coastal Basin Environm, Fuqing 350300, Peoples R China
[3] Dongying Huize Agr Technol Co Ltd, Dongying 257503, Peoples R China
[4] Shanghai Ocean Univ, Shanghai Collaborat Innovat Ctr Aquat Anim Genet &, Shanghai 201306, Peoples R China
[5] Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China
[6] Shenzhen Alpha Feed Co Ltd, Shenzhen 518054, Peoples R China
来源
ANIMALS | 2024年 / 14卷 / 22期
关键词
SSR; Chinese mitten crab; multiplex PCR; family selection; genetic diversity; parentage assignment; CHINESE MITTEN CRAB; GENETIC DIVERSITY; PARENTAGE DETERMINATION; POPULATION-STRUCTURE; COMPUTER-PROGRAM; LOCI; IDENTIFICATION; PATERNITY;
D O I
10.3390/ani14223200
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Background: The development of genetic markers is crucial for aquaculture and population genetics. Polyallelic microsatellite markers are widely used for breeding and family management of Eriocheir sinensis (E. sinensis), contributing to the sustainable development of the industry. Methods: Through transcriptome analysis, 100 highly polymorphic SSR markers were identified and developed. From these, four multiplex PCR panels, each containing 16 markers, were constructed and applied to parentage assignment in six E. sinensis families. Results: The number of alleles (Na) ranged from 7 to 21, the observed heterozygosity (Ho) varied from 0.356 to 0.951, and the expected heterozygosity (He) varied from 0.656 to 0.909. The polymorphism information content (PIC) ranged from 0.633 to 0.900. The CERVUS analysis showed that using only the two most informative multiplex PCR panels, assignment success rates exceeded 90.34%, with 100% accuracy in assigning offspring to parents when three or four panels were used. Conclusions: This method significantly enhances the efficiency of microsatellite marker analysis, which provides a convenient and cost-effective method for breeding programs and family management of E. sinensis.
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页数:14
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