Enhanced methanol-xylose co-utilization strategy in Komagataella phaffii

Bio-manufacturing based on non-food carbon sources is conducive to alleviating the global food crisis and greenhouse effect. However, the mechanism of the utilization of methanol and xylose in Komagataella phaffii based on endogenous metabolic pathways has not been fully explored. In this study, transcriptomics revealed a positive correlation between methanol metabolic efficiency and the transcription level of genes related to xylose metabolism and phosphate metabolism. By providing sufficient phosphate to the strain, the methanol utilization rate of the Komagataella phaffii GA01 strain was improved, and the final biomass reached 7.5 g DCW/L. Metabolomics further confirmed that methanol could effectively activate xylose metabolism of the strain, and the consumption rates of methanol and xylose of the Komagataella phaffii GA01 strain could reach 3.87 g/L/d and 1.83 g/L/d, which were 34 % and 357.5 % higher than that of the wild-type strain, respectively. This study further promotes the application of methanol and xylose in microbial fermentation.