Essential oil active constituents loaded PVA nanofibers enhance antibiofilm activity against Candida albicans and Candida tropicalis

被引:2
|
作者
Chaudhary, Pankaj Kumar [1 ]
Saini, Deepika [2 ]
Mishra, Purusottam [1 ]
Pandav, Kumud [1 ]
Prasad, Ramasare [1 ]
机构
[1] Indian Inst Technol Roorkee, Dept Biosci & Bioengn, Mol Biol & Prote Lab, Roorkee 247667, Uttarakhand, India
[2] All India Inst Med Sci AIIMS Rishikesh, Dept Biochem, Rishikesh 249203, India
关键词
Biofilm; PVA nanofiber; Limonene and linalool; Encapsulation; Therapeutic intervention; MEMBRANE;
D O I
10.1016/j.jddst.2024.105871
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Candida species cause infections in immunocompromised patients, making them resistant against available antifungal drugs. Therefore, developing new therapeutic intervention to prevent biofilm formation with minimal toxicity is urgently needed. The present study aims to improve the anti-biofilm formation potential of terpenes (limonene/linalool) by nanofiber encapsulation using electrospinning technique. Furthermore, nanofibers were characterized using an array of biophysical tools such as FESEM, FTIR, TGA/DTG, XRD, UV-Vis spectroscope, NMR, water contact angle. The average diameter was 175.54 +/- 41.61 nm and 158.37 +/- 40.32 nm for EO-ACs/ PVA-NF and PVA-NF respectively. The antibiofilm activity of EO-ACs nanofibers were evaluated by MTT assay. The data illustrate that EO-ACs loaded NF3 eradicate the Candida biofilm (>90%) and microscopic evidence also supported the MTT results. The cytotoxicity of EO-ACs/PVA-NF was found negligible to human embryonic kidney cells (HEK-293) and >80 % of cells were live.
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页数:13
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