Protective effects of bone marrow mesenchymal stem cell-derived exosomes loaded cerium dioxide nanoparticle against deoxynivalenol-induced liver damage

Background Deoxynivalenol (DON), a mycotoxin produced by Fusarium species, posed significant threats to food safety and human health due to its widespread prevalence and detrimental effects. Upon exposure, the liver, which played a crucial role in detoxifying DON, experienced depleted antioxidant levels and heightened inflammatory responses. Bone marrow mesenchymal stem cell (BMSC)-derived exosomes (BMSC-exos) exhibited therapeutic potential by promoting cellular repair and delivering bioactive substances, such as cerium dioxide nanoparticles (CeO2 NPs), which are recognized for their ability to mitigate oxidative stress and inflammation. Results We successfully loaded BMSC-exos with CeO2 NPs (BMSC-exos @ CeO2 ) using extrusion techniques, verified through electron microscopy and elemental mapping. The resulting BMSC-exos @ CeO2 displayed low cytotoxicity, boosted antioxidant activity, and reduced inflammation in Hepa 1-6 cells with DON condition. In vivo study, BMSC-exos @ CeO2 maintained stability for 72 h, it also can prevent antioxidant depletion and inhibit liver inflammation under the DON condition. After BMSC-exos @ CeO2 treatment, multi-omics analyses further highlighted significant changes in metabolic and protein signaling pathways, notably in linoleic and arachidonic acid metabolism. Key pathways about AMPK and JAK1/STAT3 were involved in mitigating liver damage with or without DON. Conclusion Our findings revealed BMSC-exos @ CeO2 as a promising therapeutic strategy against DON's toxicity, offering valuable insights into their potential for liver protection.