Doxycycline-integrated silk fibroin hydrogel: preparation, characterizations, and antimicrobial assessment for biomedical applications

被引:0
|
作者
Ealla, Kranti Kiran Reddy [1 ,2 ]
Durga, Chandra Sri [3 ]
Sahu, Vikas [2 ]
Kumari, Neema [4 ]
Venkatesan, Jayachandran [5 ]
Veeraraghavan, Vishnu Priya [6 ]
Ramani, Pratibha [7 ]
Bokara, Kiran Kumar [8 ]
Ramalingam, Karthikeyan [2 ]
机构
[1] Saveetha Univ, Saveetha Inst Med & Tech Sci, Dept Oral & Maxillofacial Pathol, Chennai 600077, India
[2] Malla Reddy Vishwavidyapeeth, Malla Reddy Inst Dent Sci, Dept Oral & Maxillofacial Pathol, Hyderabad 500055, India
[3] Aasya Hlth Care Pvt Ltd, Atal Incubat Ctr, Ctr Cellular & Mol Biol, Hyderabad 500039, India
[4] Malla Reddy Inst Med Sci, Dept Microbiol, Hyderabad 500055, India
[5] Malla Reddy Vishwavidyapeeth, Sch Dent Sci & Technol, Dept Biomat & Transplant Sci, Hyderabad 500055, Telangana, India
[6] Saveetha Univ, Saveetha Inst Med & Tech Sci, Ctr Mol Med & Diagnost CoMManD, Chennai 600077, India
[7] Saveetha Univ, Saveetha Dent Coll & Hosp, Dept Oral & Maxillofacial Pathol, Chennai 600077, India
[8] CSIR Ctr Cellular & Mol Biol, Annexe II, Med Biotechnol Complex, Uppal Rd, Hyderabad 500007, Telangana, India
来源
BMC ORAL HEALTH | 2025年 / 25卷 / 01期
关键词
Antimicrobials; Biocompatible; Controlled drug delivery; Doxycycline; Hydrogel; Silk fibroin; IN-SITU GEL; SCAFFOLDS; MICROPARTICLES; NANOPARTICLES; FORMULATION; HYCLATE; RELEASE; GROWTH;
D O I
10.1186/s12903-025-05568-4
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
BackgroundTooth extraction, a common dental procedure, is often accompanied by pain, trismus, and swelling due to alveolitis caused by oral bacteria. Doxycycline is prescribed to alleviate infection and improve socket healing, but its immediate absorption in the bloodstream makes the treatment less effective at oral sites. This emphasizes the importance of a drug delivery system to gradually slow its release at the oral wound site and increase its bioavailability to make the treatment effective over time.MethodsSilk fibroin (SF) - doxycycline hyclate (DH) hydrogel was developed and subsequently characterized for gelation kinetics, swelling, stress-strain analysis, morphology using scanning electron microscopy, interaction between SF and DH using Fourier transform infrared (FT-IR) spectroscopy, drug release profile, antibacterial efficacy, and biocompatibility studies.ResultsThe results indicated that the SF-DH hydrogel maintained its structural integrity, tolerated stress and strain, and featured interconnected pores, confirming DH integration within the SF matrix. The SF-DH hydrogel formed within 8 h with the pore size range of 20-150 mu m and 90.72 kPa Young's modulus. The drug release profile showed the increased release of DH up to 2 h, followed by sustained release till 8 h. The zone of inhibition was smaller with SF-DH hydrogels compared with DH for both Staphylococcus aureus and Streptococcus mutans. Furthermore, MC3T3-E1 cells showed 90% viability with SF-DH hydrogel.ConclusionsThe findings suggest that SF-DH hydrogel showed sufficient mechanical strength, pore size, antimicrobial activity, and biocompatibility. Further in vivo and clinical tests are required to prove its efficacy in effective socket healing.
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页数:10
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