Chromosome-level and haplotype-resolved genome assembly of Bougainvillea glabra

被引:0
|
作者
Lan, Lan [1 ,2 ,3 ]
Li, Haiyan [1 ,4 ]
Xu, Shisong [1 ,4 ]
Xu, Yueting [1 ,5 ]
Leng, Qingyun [1 ,4 ]
Zhang, Linbi [6 ]
Wu, Linqiao [7 ]
Yin, Junmei [1 ,4 ,8 ]
Wu, Zhiqiang [2 ]
Niu, Junhai [1 ,4 ]
机构
[1] Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Natl Key Lab Trop Crop Breeding, Key Lab Gene Resources & Germplasm Enhancement Sou, Haikou, Peoples R China
[2] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Guangdong Lab Lingnan Modern Agr, Genome Anal Lab,Minist Agr & Rural Affairs,Shenzhe, Shenzhen, Peoples R China
[3] Murdoch Univ, Coll Sci Hlth Engn & Educ, State Agr Biotechnol Ctr SABC, Perth, WA 6150, Australia
[4] Engn Technol Res Ctr Trop Ornamental Plant Germpla, Danzhou, Peoples R China
[5] Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China
[6] Hainan Daxin Landscape Co Ltd, Haikou, Peoples R China
[7] Hainan Big Lake Bridge Technol Co Ltd, Haikou, Peoples R China
[8] Chinese Acad Trop Agr Sci, Sanya Res Inst, Sanya, Peoples R China
关键词
HI-C; ALIGNMENT;
D O I
10.1038/s41597-024-04333-6
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Bougainvillea glabra Choisy, a common perennial shrub or climbing vine in tropical and subtropical areas, has significant ornamental and ecological value due to its showy floriferous blooms and adaptability to multiple environmental stresses. In this study, based on PacBio and Hi-C sequencing data, we presented the haplotype-resolved, and chromosome-level assembled genomes for B. glabra (2n = 34). We obtained two haplotypes with genome size of 2,855,692,602 bp and 2,889,511,499 bp, N50 of 165 Mbp and 167 Mbp, and both anchored to 17 chromosomes with anchor ratio of 97.07% and 97.55%, respectively. Through the BUSCO evaluation process, the two assembled haplotypes both exhibited the high score of 96%. By combining the high-confidence different evidence of genome structure annotation pipeline we annotated each haplotype containing similar to 33,000 predicted genes, of which 86% were functionally annotated by different databases. These high-quality haplotypes assembly and annotation results will serve as a great genomic resource to explore the ornamental traits and environmental adaptability and accelerate improvement in future.
引用
收藏
页数:7
相关论文
共 50 条
  • [41] Chromosome-level scaffolding of haplotype-resolved assemblies using Hi-C data without reference genomes
    Zeng, Xiaofei
    Yi, Zili
    Zhang, Xingtan
    Du, Yuhui
    Li, Yu
    Zhou, Zhiqing
    Chen, Sijie
    Zhao, Huijie
    Yang, Sai
    Wang, Yibin
    Chen, Guoan
    NATURE PLANTS, 2024, 10 (08) : 1184 - 1200
  • [42] Haplotype-resolved de novo assembly of the Vero cell line genome
    Sene, Marie-Angelique
    Kiesslich, Sascha
    Djambazian, Haig
    Ragoussis, Jiannis
    Xia, Yu
    Kamen, Amine A.
    NPJ VACCINES, 2021, 6 (01)
  • [43] Haplotype-resolved de novo assembly of the Vero cell line genome
    Marie-Angélique Sène
    Sascha Kiesslich
    Haig Djambazian
    Jiannis Ragoussis
    Yu Xia
    Amine A. Kamen
    npj Vaccines, 6
  • [44] Construction and analysis of the chromosome-level haplotype-resolved genomes of two Crassostrea oyster congeners: Crassostrea angulata and Crassostrea gigas
    Qi, Haigang
    Cong, Rihao
    Wang, Yanjun
    Li, Li
    Zhang, Guofan
    GIGASCIENCE, 2023, 12
  • [45] A chromosome-level genome assembly of Plantago ovata
    Herliana, Lina
    Schwerdt, Julian G.
    Neumann, Tycho R.
    Severn-Ellis, Anita
    Phan, Jana L.
    Cowley, James M.
    Shirley, Neil J.
    Tucker, Matthew R.
    Bianco-Miotto, Tina
    Batley, Jacqueline
    Watson-Haigh, Nathan S.
    Burton, Rachel A.
    SCIENTIFIC REPORTS, 2023, 13 (01):
  • [46] Chromosome-level genome assembly of the cashmere goat
    Wang, Zhiying
    Lv, Qi
    Li, Wenze
    Huang, Wanlong
    Gong, Gao
    Yan, Xiaochun
    Liu, Baichuan
    Chen, Oljibilig
    Wang, Na
    Zhang, Yanjun
    Wang, Ruijun
    Li, Jinquan
    Tian, Shilin
    Su, Rui
    SCIENTIFIC DATA, 2024, 11 (01)
  • [47] A chromosome-level genome assembly of Plantago ovata
    Lina Herliana
    Julian G. Schwerdt
    Tycho R. Neumann
    Anita Severn-Ellis
    Jana L. Phan
    James M. Cowley
    Neil J. Shirley
    Matthew R. Tucker
    Tina Bianco-Miotto
    Jacqueline Batley
    Nathan S. Watson-Haigh
    Rachel A. Burton
    Scientific Reports, 13 (1)
  • [48] Chromosome-level genome assembly of Salvia sclarea
    Choi, Sehyun
    Kang, Yuna
    Kim, Changsoo
    SCIENTIFIC DATA, 2025, 12 (01)
  • [49] Chromosome-level genome assembly of Platycarya strobilacea
    Zhou, Huijuan
    Zhang, Xuedong
    Liu, Hengzhao
    Ma, Jiayu
    Hao, Fan
    Ye, Hang
    Wang, Yaling
    Zhang, Shuoxin
    Yue, Ming
    Zhao, Peng
    SCIENTIFIC DATA, 2024, 11 (01)
  • [50] Chromosome-level genome assembly of Ajuga decumbens
    Gao, Yubang
    Li, Jingzhao
    Xie, Yuli
    Zhang, Teng
    Tian, Kai
    Li, Xiaotang
    Yao, Lunguang
    FRONTIERS IN PLANT SCIENCE, 2024, 15