Detection of Theileria annulata carrier cattle by PCR

被引：0
|
作者：
Roy, K.C.
Ray, D.
Bansal, G.C.
Singh, R.K.
机构：
来源：
Indian Journal of Experimental Biology | 2000年 / 38卷 / 03期
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A simple method for treating bovine blood samples for direct detection of T. annulata in carriers, after polymerase chain reaction (PCR) amplification of small subunit ribosomal RNA (SSU rRNA) gene is described. The threshold of detection of the PCR-assay was an erythrocytic parasitaemia of 0.00008% corresponding to 16 infected bovine erythrocytes. In 50 known carriers, 42 were positive in PCR, in which 8 cattle revealed presence of T. annulata in stained blood smear under microscope.
引用
收藏
页码：283 / 284
相关论文
共 50 条
  • [21] Theileria annulata:: Carrier state and immunity
    Ilhan, T
    Williamson, S
    Kirvar, E
    Shiels, B
    Brown, CGD
    TROPICAL VETERINARY MEDICINE: MOLECULAR EPIDEMIOLOGY, HEMOPARASITES AND THEIR VECTORS, AND GENERAL TOPICS, 1998, 849 : 109 - 125
  • [22] Field evaluation of PCR in detecting Theileria annulata infection in cattle in eastern Turkey
    Aktas, M
    Dumanli, N
    Çetinkaya, B
    Çakmak, A
    VETERINARY RECORD, 2002, 150 (17) : 548 - 549
  • [23] Nested PCR assay for detection of Theileria annulata in Hyalomma anatolicum infesting cattle from coastal Odisha, India
    Dehuri, Manaswini
    Panda, Mitraranjan
    Sahoo, Niranjan
    Mohanty, Bijayendranath
    Behera, Bikash
    ANIMAL BIOTECHNOLOGY, 2022, 33 (06) : 1229 - 1234
  • [24] Pentaplex PCR assay for rapid differential detection of Babesia bigemina, Theileria annulata, Anaplasma marginale and Trypanosoma evansi in cattle
    Ganguly, Anita
    Maharana, Biswa Ranjan
    Ganguly, Indrajit
    BIOLOGICALS, 2020, 63 : 81 - 88
  • [25] BIOCHEMICAL VALUES IN CATTLE INFECTED WITH THEILERIA-ANNULATA
    RAYULU, VC
    HAFEEZ, M
    INDIAN VETERINARY JOURNAL, 1995, 72 (11): : 1208 - 1209
  • [26] IMMUNOLOGICAL ASPECTS OF THEILERIA-ANNULATA INFECTIONS OF CATTLE
    AHMED, JS
    DEUTSCHE TIERARZTLICHE WOCHENSCHRIFT, 1986, 93 (07): : 342 - 342
  • [27] EFFECTIVENESS OF PARVAQUONE IN CATTLE INFECTED WITH THEILERIA-ANNULATA
    UNSUREN, H
    KURTDEDE, A
    GOKSU, K
    TROPICAL ANIMAL HEALTH AND PRODUCTION, 1988, 20 (04) : 256 - 258
  • [28] HEMATOLOGICAL VALUES IN CATTLE INFECTED WITH THEILERIA-ANNULATA
    RAYULU, VC
    HAFEEZ, MD
    INDIAN JOURNAL OF ANIMAL SCIENCES, 1995, 65 (11): : 1202 - 1203
  • [29] Comparison of molecular and microscopic technique for detection of Theileria annulata from the field cases of cattle
    Chauhan, H. C.
    Patel, B. K.
    Bhagat, A. G.
    Patel, M. V.
    Patel, S. I.
    Raval, S. H.
    Panchasara, H. H.
    Shrimali, M. D.
    Patel, A. C.
    Chandel, B. S.
    VETERINARY WORLD, 2015, 8 (11) : 1370 - 1374
  • [30] Naturally occurring infections of cattle with Theileria lestoquardi and sheep with Theileria annulata in the Sudan
    Taha, K. M.
    Salih, D. A.
    Ali, A. M.
    Omer, R. A.
    El Hussein, A. M.
    VETERINARY PARASITOLOGY, 2013, 191 (1-2) : 143 - 145
12345