Wide-field optically sectioning fluorescence microscopy with laser illumination

被引：67
|
作者：
Neil, M.A.A. [1 ]
Squire, A. [1 ,2 ]
Juškaitis, R. [1 ]
Bastiaens, P.I.H. [1 ,2 ]
Wilson, T. [1 ]
机构：
[1] Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, United Kingdom
[2] Cell Biophysics Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, United Kingdom
来源：
Journal of Microscopy | 2000年 / 197卷 / 01期
关键词：
Fluorescence;
D O I：
10.1046/j.1365-2818.2000.00656.x
中图分类号：
学科分类号：
摘要：
We describe an extremely simple method by which optically sectioned fluorescence images may be obtained with conventional microscopes using laser illumination. A one-dimensional grid pattern is introduced into the illumination system, together with a rotating ground glass diffuser. This causes an image of the grid pattern to be projected into the specimen. Images taken at three spatial positions of the grid are processed in a simple manner to provide optically sectioned images of fluorescent specimens.
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页码：1 / 4
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