Wide-field optically sectioning fluorescence microscopy with laser illumination

被引：67
|
作者：
Neil, M.A.A. [1 ]
Squire, A. [1 ,2 ]
Juškaitis, R. [1 ]
Bastiaens, P.I.H. [1 ,2 ]
Wilson, T. [1 ]
机构：
[1] Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, United Kingdom
[2] Cell Biophysics Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, United Kingdom
来源：
Journal of Microscopy | 2000年 / 197卷 / 01期
关键词：
Fluorescence;
D O I：
10.1046/j.1365-2818.2000.00656.x
中图分类号：
学科分类号：
摘要：
We describe an extremely simple method by which optically sectioned fluorescence images may be obtained with conventional microscopes using laser illumination. A one-dimensional grid pattern is introduced into the illumination system, together with a rotating ground glass diffuser. This causes an image of the grid pattern to be projected into the specimen. Images taken at three spatial positions of the grid are processed in a simple manner to provide optically sectioned images of fluorescent specimens.
引用
收藏
页码：1 / 4
相关论文
共 50 条
  • [1] Wide-field optically sectioning fluorescence microscopy with laser illumination
    Neil, MAA
    Squire, A
    Juskaitis, R
    Bastiaens, PIH
    Wilson, T
    JOURNAL OF MICROSCOPY-OXFORD, 2000, 197 : 1 - 4
  • [2] Wide-field fluorescence sectioning microscopy using dynamic speckle illumination
    Yin, Jun
    Qu, Junle
    Shao, Yonghong
    Zhao, Lingling
    Lin, Haoming
    Niu, Hanben
    OPTICS IN HEALTH CARE AND BIOMEDICAL OPTICS III, 2008, 6826
  • [3] Study on wide-field fluorescence sectioning microscopy based on dynamic speckle illumination
    Lin Hao-Ming
    Shao Yong-Hong
    Qu Jun-Le
    Yin Jun
    Chen Si-Ping
    Niu Han-Ben
    ACTA PHYSICA SINICA, 2008, 57 (12) : 7641 - 7649
  • [4] Wide-field fluorescence sectioning with hybrid speckle and uniform-illumination microscopy
    Lim, Daryl
    Chu, Kengyeh K.
    Mertz, Jerome
    OPTICS LETTERS, 2008, 33 (16) : 1819 - 1821
  • [5] Study on wide-field fluorescence sectioning microscopy based on dynamic speckle illumination
    Lin, Hao-Ming
    Shao, Yong-Hong
    Qu, Jun-Le
    Yin, Jun
    Chen, Si-Ping
    Niu, Han-Ben
    Wuli Xuebao/Acta Physica Sinica, 2008, 57 (12): : 7641 - 7649
  • [6] Optically sectioned wide-field fluorescence lifetime imaging microscopy enabled by structured illumination
    Hinsdale, Taylor
    Olsovsky, Cory
    Rico-Jimenez, Jose J.
    Maitland, Kristen C.
    Jo, Javier A.
    Malik, Bilal H.
    BIOMEDICAL OPTICS EXPRESS, 2017, 8 (03): : 1455 - 1465
  • [7] Wide-field depth-sectioning fluorescence microscopy using projector-generated patterned illumination
    Delica, Serafin
    Blanca, Carlo Mar
    APPLIED OPTICS, 2007, 46 (29) : 7237 - 7243
  • [8] Image formation in structured illumination wide-field fluorescence microscopy
    Karadaglic, Dejan
    Wilson, Tony
    MICRON, 2008, 39 (07) : 808 - 818
  • [9] Time-resolved wide-field optically sectioned fluorescence microscopy
    Dupuis, Guillaume
    Benabdallah, Nadia
    Chopinaud, Aurelien
    Mayet, Celine
    Leveque-Fort, Sandrine
    THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING XX, 2013, 8589
  • [10] Wide-field Fourier ptychographic microscopy using laser illumination source
    Chung, Jaebum
    Lu, Hangwen
    Ou, Xiaoze
    Zhou, Haojiang
    Yang, Changhuei
    BIOMEDICAL OPTICS EXPRESS, 2016, 7 (11): : 4787 - 4802
12345