Improving osteoblast functions and bone formation upon BMP-2 immobilization on titanium modified with heparin

被引：0
|
作者：
Kim, Sung Eun [1 ]
Kim, Chang-Seop [2 ]
Yun, Young-Pil [1 ]
Yang, Dae Hyeok [3 ]
Park, Kyeongsoon [4 ]
Kim, Se Eun [5 ]
Jeong, Chang-Mo [2 ]
Huh, Jung-Bo [2 ]
机构：
[1] Department of Orthopedic Surgery, Korea University Medical Center, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul,152-703, Korea, Republic of
[2] Department of Prosthodontics, School of Dentistry, Pusan National University, Yang San,626-787, Korea, Republic of
[3] Institute of Cell and Tissue Engineering, College of Medicine, Catholic University of Korea, Seoul,137-701, Korea, Republic of
[4] Division of Bio-Imaging, Chuncheon Center, Korea Basic Science Institute, 192-1, Hyoja 2-dong, Chuncheon, Gangwon-do,200-701, Korea, Republic of
[5] Department of Veterinary Surgery, College of Veterinary Medicine, Chonnam National University, Gwangju,500-757, Korea, Republic of
来源：
Carbohydrate Polymers | 2014年 / 114卷
基金：
新加坡国家研究基金会;
关键词：
Bone density - Bone formation - Bone morphogenetic protein-2 - Bone-to-implant contact - Calcium deposition - Delivery systems - Heparin - Osteoblast function;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The aim of this study was to develop bone morphogenetic protein-2 (BMP-2) immobilization on titanium (Ti) modified by heparin for improving osteoblast function in vitro and bone formation in vivo. The Ti surface was first modified with heparin and then immobilized with BMP-2 (BMP-2/Hep-Ti). The Ti and modified Ti were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and contact angle. In vitro studies demonstrated that osteoblasts cultured on BMP-2/Hep-Ti substrate increased ALP activity, calcium deposition, osteocalcin (OCN) and osteopontin (OPN) levels as compared to those cultured on Ti or BMP-2/Ti. In addition, intra-thread bone density and bone to implant contact ratio of BMP-2/Hep-Ti were significantly greater than those of Ti in the in vivo study. In conclusion, BMP-2 immobilized Ti modified heparin implants seemed to be a suitable delivery system for BMP-2 to improve osteoblast functions and new bone formation at the defect area around an implant. © 2014 Elsevier Ltd. All rights reserved.
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页码：123 / 132
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