Probing structure in invisible protein states with anisotropic NMR chemical shifts

被引：0

作者：

Vallurupalli, Pramodh ^{[1
]}

Hansen, D. Flemming ^{[1
]}

Kay, Lewis E. ^{[1
]}

机构：

[1] Departments of Molecular Genetics, Biochemistry, and Chemistry, University of Toronto, Toronto, ON M5S 1A8, Canada

来源：

Journal of the American Chemical Society | 2008年 / 130卷 / 09期

关键词：

A general method for obtaining quantitative structural information on invisible; excited protein states by solution-based NMR spectroscopy is presented. The approach exploits relaxation dispersion techniques in which changes in chemical shifts between ground and excited states are monitored in solutions with and without small amounts of residual molecular alignment. This allows the calculation of differences in chemical shifts induced by alignment that can be directly related to molecular structure; in cases where the orientation and magnitude of the chemical-shift tensor are well defined. An example using carbonyl chemical shifts as probes of a protein-ligand binding reaction is presented to illustrate and validate the method. Copyright © 2008 American Chemical Society;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Journal article (JA)

引用

页码：2734 / 2735

共 50 条

[31] HYDROPHOBIC CLUSTERING IN NONNATIVE STATES OF A PROTEIN - INTERPRETATION OF CHEMICAL-SHIFTS IN NMR-SPECTRA OF DENATURED STATES OF LYSOZYME
EVANS, PA
TOPPING, KD
WOOLFSON, DN
DOBSON, CM
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 1991, 9 (04) : 248 - 266
[32] Accurate Measurement of Alpha Proton Chemical Shifts of Excited Protein States by Relaxation Dispersion NMR Spectroscopy
Lundstroem, Patrik
Hansen, D. Flemming
Vallurupalli, Pramodh
Kay, Lewis E.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (05) : 1915 - 1926
[33] Accurate measurement of alpha proton chemical shifts of excited protein states by relaxation dispersion NMR spectroscopy
Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada
J. Am. Chem. Soc., 2009, 5 (1915-1926):
[34] PROBING PROTEIN-STRUCTURE AND DYNAMICS BY TRITIUM NMR
OCONNELL, TM
WILLIAMS, PG
GERIG, JT
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS, 1993, 33 (05): : 371 - 380
[35] Structures of invisible, excited protein states by relaxation dispersion NMR spectroscopy
Vallurupalli, Pramodh
Hansen, D. Flemming
Kay, Lewis E.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (33) : 11766 - 11771
[36] Interpreting Protein Structural Dynamics from NMR Chemical Shifts
Robustelli, Paul
Stafford, Kate A.
Palmer, Arthur G., III
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (14) : 6365 - 6374
[37] A robust algorithm for optimizing protein structures with NMR chemical shifts
Berjanskii, Mark
Arndt, David
Liang, Yongjie
Wishart, David S.
JOURNAL OF BIOMOLECULAR NMR, 2015, 63 (03) : 255 - 264
[38] A robust algorithm for optimizing protein structures with NMR chemical shifts
Mark Berjanskii
David Arndt
Yongjie Liang
David S. Wishart
Journal of Biomolecular NMR, 2015, 63 : 255 - 264
[39] Prediction of the Structure of Nobilisitine A Using Computed NMR Chemical Shifts
Lodewyk, Michael W.
Tantillo, Dean J.
JOURNAL OF NATURAL PRODUCTS, 2011, 74 (05): : 1339 - 1343
[40] PROTEIN-STRUCTURE REFINEMENT AND PREDICTION VIA NMR CHEMICAL-SHIFTS AND QUANTUM-CHEMISTRY
LE, HB
PEARSON, JG
DEDIOS, AC
OLDFIELD, E
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (13) : 3800 - 3807

← 1 2 3 4 5 →