Utility of 4-chloro-7-nitrobenzofurazan (NBD-Cl) for the spectrophotometric and spectrofluorometric determination of several antihistamine and antihypertensive drugs

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[1] [1,El-Hay, Soad S. Abd
[2] Colyer, Christa L.
[3] Hassan, Wafaa S.
[4] Shalaby, Abdalla
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El-Hay, S.S.A. (soadselem@yahoo.co.uk) | 1600年 / AOAC International卷 / 96期
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New; sensitive; and selective spectrophotometric and spectrofluorometric methods have been developed for determination of clemastine hydrogen fumarate (Clem); loratadine; (Lor); losartan potassium (Los); and ramipril (Ram) in both pure form and pharmaceutical formulations using 4-chloro-7-nitrobenzofurazan (NBD-Cl); which is a highly sensitive chromogenic and fluorogenic reagent. The relation between absorbance at 470; 467; 471; and 469 nm and the concentration was linear over the ranges 5-35; 10-100; 10-90; and 10-120 μg/mL for Clem; Lor; Los; and Ram; respectively. The complexation products were also measured spectrofluorometrically at the emission wavelength 535 nm for Clem; and Ram and at 538 nm for Los with excitation at 477 and 452 nm for Clem and Lor; respectively; and 460 nm for both Los and Ram. The fluorescence intensity was directly proportional to the drug concentration over the ranges 0.05-0.5; 5-20; 1-6; and 2-15 μg/mL for Clem; respectively. The methods were successfully applied for the determination of the studied drugs in pharmaceutical dosage forms with excellent recovery. © 2014 Publishing Technology;
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