High-speed atomic force microscopy for studying the dynamic behavior of protein molecules at work

被引：0

作者：

Ando, Toshio ^{[1
,2
,3
]}

Uchihashi, Takayuki ^{[1
,3
]}

Kodera, Noriyuki ^{[1
]}

Miyagi, Atsushi ^{[1
]}

Nakakita, Ryo ^{[1
]}

Yamashita, Hayato ^{[1
]}

Sakashita, Mitsuru ^{[1
]}

机构：

[1] Department of Physics, Faculty of Science, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan

[2] Frontier Science Research Organization, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan

[3] JST/CREST, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

来源：

Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers | 2006年 / 45卷 / 3 B期

关键词：

In the quest for the mechanism of protein functions; various key techniques and instruments have been developed. This is an era when scrutinizing a certain protein from various angles is becoming possible through combined knowledge of its structure and function. However; it is necessary to link these different aspects of a protein along a time axis; but no technology is available for tracing a protein in action; at high spatial and temporal resolutions. Atomic force microscopy made it possible for the first time to view a nanometer-scale world in an aqueous environment. In 2001; we developed the first-generation high-speed atomic force microscope (AFM) that could capture moving protein molecules on video at 80 ms/frame. Since then; we have been carrying out various efforts to increase its scan rate as well as to substantially reduce tip-sample interaction force. The reduction in this force is a key to making the high-speed AFM practically useful in life sciences. Various new techniques and devices developed in the past four years have brought the AFM to its second-generation stage. It can now capture weakly interacting protein molecules successively without disturbing their physiological function. Here; we report our efforts made over the past four years; the present capacity of the high-speed AFM; and our preliminary work on the next generation of the instrument. © 2006 The Japan Society of Applied Physics;

D O I：

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摘要：

Journal article (JA)

引用

页码：1897 / 1903

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