A low-cost homogenizing device for in-field and remote DNA and RNA extraction

被引:0
|
作者
Schang, Christelle [1 ]
Zhang, Le [1 ]
Shi, Baiqian [1 ]
Nolan, Monica [1 ]
Poon, Rachael [2 ]
McCarthy, David T. [1 ,3 ,4 ]
机构
[1] Monash Univ, Dept Civil Engn, BoSL Water Monitoring & Control, Melbourne, Vic 3800, Australia
[2] Dept Hlth Victoria, Melbourne, Vic 3000, Australia
[3] QUT, Sch Civil & Environm Engn, Brisbane, Qld 4001, Australia
[4] Univ Guelph, Ontario Agr Coll, Sch Environm Sci, Guelph, ON, Canada
来源
HARDWAREX | 2024年 / 20卷
基金
澳大利亚研究理事会;
关键词
3D printing; Bead beater; Remote processing; Wastewater-based epidemiology; WBE; Portable; Environmental sampling; Specifications table; WATER;
D O I
10.1016/j.ohx.2024.e00592
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
Environmental monitoring of microorganisms is critical for the protection and enhancement of human and ecosystem health. Even though these molecular methods have overtaken traditional culture-based methods and become more accessible, these techniques still require expensive equipment and dedicated facilities to process samples which in the context of a global pandemic, remote sampling areas or low-income countries can be extremely challenging. Sample preparation and sample homogenisation are critical steps for molecular-based techniques, especially for the extraction of DNA and RNA. This study developed a low-cost, open-source, freely available 3D printed homogenizer for the processing of DNA and RNA extraction. The BoSL Beater 3D is a portable device that allows researcher to perform bead-beating steps commonly required for environmental sample extraction protocols in the field and without access to main's power. The BoSL Beater 3D was tested on filtered wastewater samples and passive samplers exposed to wastewater over a 24-hour period and showed similar or better performance to the traditional laboratory bead beater for both the extraction of DNA and RNA. The cost of this 3D homogeniser is roughly $18 AUD ($296 AUD with the jigsaw, which is roughly 57 times cheaper than a traditional bead beater) and has the added usability of being portable and easily adaptable to any type of jigsaw. In combination to newly developed field extraction kits as well as portable PCR machines, this 3D homogeniser could provide the tool necessary to enable access to molecular testing in remote setting as well as developing countries, which may not have access to fully equipped laboratories, but also allow for timely reporting. In addition, the BoSL Beater 3D, in combination with field extraction kit, can allow more flexibility to researchers while sampling, shipping, and processing DNA and RNA samples, whilst maintaining quality of these samples.
引用
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页数:13
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