Microbial community dynamics during assays of harbour oil spill bioremediation: A microscale simulation study

被引：0
|
作者：
Cappello, S. [1 ,4 ]
Caruso, G. [1 ]
Zampino, D. [2 ]
Monticelli, L.S. [1 ]
Maimone, G. [1 ]
Denaro, R. [1 ]
Tripodo, B. [1 ]
Troussellier, M. [3 ]
Yakimov, M. [1 ]
Giuliano, L. [1 ]
机构：
[1] Istituto Per l'Ambiente Marino Costiero (IAMC) IST-CNR, Section of Messina, Messina, Italy
[2] Istituto di Chimica e di Tecnologia dei Polimeri (ICTP) CNR, Section of Catania, Catania, Italy
[3] Laboratoire Ecosystèmes Lagunaires UMR-CNRS 5119, Equipe EMMA, Université de Montpellier II, Montpellier, France
[4] Istituto Per l'Ambiente Marino Costiero IST-CNR, Section of Messina, Italy
来源：
Journal of Applied Microbiology | 2007年 / 102卷 / 01期
关键词：
Aims: Microcosm experiments simulating an oil spill event were performed to evaluate the response of the natural microbial community structure of Messina harbour seawater following the accidental load of petroleum. Methods and Results: An experimental harbour seawater microcosm; supplemented with nutrients and crude oil; was monitored above 15 days in comparison with unpolluted ones (control microcosms). Bacterial cells were counted with a Live/Dead BacLight viability kit; leucine aminopeptidase; β-glucosidase; alkaline phosphatase; lipase and esterase enzymes were measured using fluorogenic substrates. The microbial community dynamic was monitored by isolation of total RNA; RT-PCR amplification of 16S rRNA; cloning and sequencing. Oil addition stimulated an increase of the total bacterial abundance; leucine aminopeptidase and phosphatase activity rates; as well as a change in the community structure. This suggested a prompt response of micro-organisms to the load of petroleum hydrocarbons. Conclusions: The present study on the viability; specific composition and metabolic characteristics of the microbial community allows a more precise assessment of oil pollution. Both structural and functional parameters offer interesting perspectives as indicators to monitor changes caused by petroleum hydrocarbons. Significance and Impact of the Study: A better knowledge of microbial structural successions at oil-polluted sites is essential for environmental bioremediation. Data obtained in microcosm studies improve our understanding of natural processes occurring during oil spills. © 2007 The Authors;
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页码：184 / 194
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