Protein crystallization by combining laser irradiation and solution-stirring techniques

被引：0

作者：

机构：

[1] [1,2,Adachi, Hiroaki

[2] 2,3,Niino, Ai

[3] 2,5,Murakami, Satoshi

[4] 2,4,Takano, Kazufumi

[5] 2,Matsumura, Hiroyoshi

[6] Kinoshita, Takayoshi

[7] Warizaya, Masaichi

[8] 2,6,Inoue, Tsuyoshi

[9] 1,2,Mori, Yusuke

[10] 1,2,Sasaki, Takatomo

来源：

Adachi, H. (adachi@pwr.eng.osaka-u.ac.jp) | 1600年 / Japan Society of Applied Physics卷 / 44期

关键词：

Crystallization - Irradiation - Laser beam effects - Neurology - Nucleation - Purification;

D O I：

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摘要：

Bovine adenosine deaminase in the absence of an inhibitor (free-ADA) does not form crystals when using conventional crystallization methods. Using a solution-stirring technique, we recently succeeded in generating a small number of free-ADA crystals. In this paper, we demonstrate the combination of laser-irradiated growth and stirring (COLAS). This technique was found to be useful for controlling crystal nucleation and growth, which led to the production of a much larger number of high-quality free-ADA crystals. © 2005 The Japan Society of Applied Physics.

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