The association of interleukin-1 gene polymorphisms with the susceptibility to chronic periodontitis in Uighur

Objective: To investigate the relationship between interleukin-1 (IL-1) gene polymorphisms and the susceptibility of chronic periodontitis in Uighur minority in Xingjiang province of China. Methods: The buccal swabs were collected from 41 severe chronic periodontitis (CP) patients, 43 moderate CP patients, 49 mild CP patients and 92 healthy controls. DNA was extracted from these buccal swabs. Genotypes of the IL-1A-889/Nco I and IL-1B+3954/Taq I were determined by sequence specific primers-polymerase chain reaction (SSP-PCR) and PCR-restriction fragment length polymorphism (PCR-RFLP). Then distribution of genotypes for IL-1A-889 and IL-1B + 3954 were compared among the different groups. Results: (1) There were no significant differences in the distribution of IL-1A-889 among severe CP patients, moderate CP patients, mild CP patients and healthy controls. (2) Frequencies of allele 2 for IL-1B+3954 were higher in severe CP patients than in healthy controls, and the difference was statistically significant. But there were no such significant differences either between moderate CP patients and healthy controls or between mild CP patients and healthy controls. Conclusion: These results suggest that IL-1B + 3953 allele 2 may be a risk indicator for the susceptibility to severe chronic periodontitis in Uighur minority in Xingjiang of China.