Molecular characteristics of ribosome DNA and genetic difference of toxigenic characteristics of food-borne alexandrium strains

被引：0
|
作者：
Gu, Lihui [1 ]
Fang, Xiang [1 ]
Zhong, Qingping [1 ]
Liao, Zhenlin [1 ]
Wang, Li [1 ]
机构：
[1] College of Food Science, South China Agricultural University, Guangzhou,510642, China
来源：
Shipin Kexue/Food Science | 2016年 / 37卷 / 15期
关键词：
DNA sequences - Polymerase chain reaction - Toxic materials - Molecular biology;
D O I：
10.7506/spkx1002-6630-201615033
中图分类号：
学科分类号：
摘要：
Paralytic shellfish poisoning could be caused by the toxin produced by Alexandrium strains, which seriously threatens human health and food safety. Molecular characteristics of ribosome DNA and toxic characteristics of foodborne Alexandrium species were analyzed by bioinformatics and computer analysis software. The 18S rDNA-28S rDNA region was amplified by PCR method, and 5.8S rDNA-ITS region was calculated by using DNAdist program. Moreover, the 18S rDNA-28S rDNA region was sequenced by PCR restricted fragment length polymorphisms (RFLP) method, and Mbo II was selected for restriction endonuclease mapping. The results showed that nucleotide sequence differences in the 5.8S rDNA-ITS region between non-toxic strain Alexandrium L35 and toxic strains could reach 0.201-0.448, while values © 2016, China Food Publishing Company. All right reserved.
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页码：198 / 203
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