Development and validation of a new RP-HPLC method for determination of quercetin in green tea

被引：0
|
作者：
机构：
[1] Savic, Ivan M.
[2] Nikolic, Vesna D.
[3] Savic, Ivana M.
[4] Nikolic, Ljubisa B.
[5] Stankovic, Mihajlo Z.
来源：
| 1600年 / Maik Nauka Publishing / Springer SBM卷 / 68期
关键词：
Green tea (Camellia sinensis) contains quercetin as a bioactive compound. Quercetin has anti-inflammatory and anticancer effects. The aim of this paper was to develop and validate an RP-HPLC method for determination of quercetin in green tea simpler and faster than other available methods. RP-HPLC analysis was performed by isocratic elution with a flow rate of 1.0 mL/min. Pure methanol was used as a mobile phase; while the quantification was effected at 370 nm. The separation was performed at 35 C using a C18 column (4.6 × 250 mm; 5 μm). The results showed that the peak area response is linear within the concentration range of 10-70 μg/mL (r = 0.9986). The values of LOD and LOQ were 1.2 and 4 μg/mL; respectively. For the intra-day and intra-instrument reproducibility; RSD were in the range of 0.05-0.84% and 0.89-1.55%; respectively. The results of accuracy for the different concentrations of quercetin (40; 50; and; 60; μg/mL); were; 101.3; 98.4; and 98.2%. The developed and validated method was successfully applied to the determination of quercetin in green tea extract. © 2013 Pleiades Publishing; Ltd;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Journal article (JA)
引用
收藏
相关论文
共 50 条
  • [41] RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF CEFPODOXIME PROXETIL
    Patle, Deepshikha
    Rote, Ambadas Rangnath
    INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH, 2014, 5 (11): : 4769 - 4773
  • [42] METHOD DEVELOPMENT AND VALIDATION FOR THE ANALYSIS OF CAPECITABINE BY RP-HPLC METHOD
    Fathima, Azka
    Gazi, Shaik
    INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2018, 5 (08): : 7808 - 7814
  • [43] DEVELOPMENT OF A NEW RP-HPLC METHOD FOR ONDANSETRON DETERMINATION IN INJECTABLE FORMULATIONS
    Balint, Alina
    Imre, Silvia
    Bondoroi-Papp, Zelma
    Muntean, Daniela-Lucia
    MEDICAL-SURGICAL JOURNAL-REVISTA MEDICO-CHIRURGICALA, 2018, 122 (01): : 207 - 212
  • [44] BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF LENVATINIB BY RP-HPLC METHOD
    Veni, G. Krishna
    Ajitha, A.
    Abbulu, K.
    INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH, 2020, 11 (07): : 3313 - 3319
  • [45] Method development and validation of asenapine in bulk by RP-HPLC method
    Aarelly, K., 1600, Journal of Chemical and Pharmaceutical Research, 3/668 Malviya Nagar, Jaipur, Rajasthan, India (04):
  • [46] Validation of method for RP-HPLC for the determination of Thiocolchicoside e in tablet
    Bor, M.
    Guilarte, A.
    Guzman, L.
    Macias, K.
    Mendoza, W.
    AFINIDAD, 2018, 75 (581) : 32 - 38
  • [47] DEVELOPMENT AND VALIDATION OF NEW RP-HPLC ASSAY METHOD FOR VALSARTAN IN PURE AND IN FORMULATIONS
    Rao, G. Sivasankara
    Raju, T. Naga T.
    Ramarao, P. V. V. S.
    Vardhan, S. V. M.
    Ramachandran, D.
    INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH, 2014, 5 (02): : 596 - 599
  • [48] Validation of method for RP-HPLC for the determination of Etoricoxib in coated tablets
    Villalba, D.
    Bor, M.
    Delgado, B.
    Sanchez, M. A.
    Zea, G.
    AFINIDAD, 2021, 78 (592) : 79 - 85
  • [49] New RP-HPLC Method Development and Validation for Dorzolamide in Ophthalmic Dosage Form
    Thangabalan, Boovizhikannan
    Kahsay, Getu
    Eticha, Tadele
    JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY, 2018, 2018
  • [50] Development and Validation of RP-HPLC Method for Simultaneous Qualitative and Quantitative Estimation of Curcumin and Quercetin in Bulk Mixture
    Patil, Nayana
    Mahajan, Hitendra
    INDIAN JOURNAL OF PHARMACEUTICAL EDUCATION AND RESEARCH, 2022, 56 (01) : 247 - 254
12345