MLST Subtyping and Virulence Genes Detection of Food-borne Listeria monocytogenes Isolates in Shanghai

被引：0
|
作者：
Cheng M. [1 ]
Wang W. [1 ]
Shi X. [1 ]
Shi C. [1 ]
机构：
[1] School of Agriculture and Biology, Shanghai Jiao Tong University, MOST-USDA Joint Research Center for Food Safety and Bor Luh Food Safety Center, Shanghai
来源：
Journal of Chinese Institute of Food Science and Technology | 2019年 / 19卷 / 02期
关键词：
Listeria monocytogenes; Multilocus sequence typing (MLST); Virulence genes;
D O I：
10.16429/j.1009-7848.2019.02.028
中图分类号：
学科分类号：
摘要：
To study the subtype and virulent gene prevalence, a total of 86 Listeria monocytogens isolates from retailed foods during June, 2013 to June, 2014 were characterized. In total, 15 MLST sequence types(STs) were identified, among which 80.2% isolates belong to Lineage II (69/86), and the others belong to Lineage I. These isolates were also characterized for the carryings of 10 virulence genes (prfA, hly, plcA, plcB, actA, clpE, mpl, inlA, inlB, and iap) by PCR. It revealed that all the isolates carried the 10 virulence genes investigated in this study. These results indicate us that there is a big risk of Listeria monocytogens to food safety existed in retailed foods in Shanghai, which calls stronger efforts on its surveillance, prevention and control. © 2019, Editorial Office of Journal of CIFST. All right reserved.
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页码：223 / 229
页数：6
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